Effect of melatonin on secreted and induced matrix metalloproteinase-9 and -2 activity during prevention of indomethacin-induced gastric ulcer
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Effect of melatonin on secreted and induced matrix
metalloproteinase-9 and -2 activity during prevention of
indomethacin-induced gastric ulcer
|
|
| Creator |
Ganguly, Krishnendu
Maity, Pallab Reiter, Russel J Swarnakar, Snehasikta |
|
| Subject |
Cell Biology & Physiology
|
|
| Description |
Matrix metalloproteinases (MMPs) maintain the crucial role in
physiological turnover of extracellular matrix (ECM) proteins in gastric
tissues. However, a little is known about the relationship of MMPs with
ECM degradation during gastric ulceration and ECM remodeling during
healing. Our objective was to investigate the effect of melatonin (N-acetyl-5
methoxytryptamine) on the regulation of MMP-9 and MMP-2 activity
during prevention of gastric ulcer. In the present study, biochemical and
zymographic methods were used to analyze the mechanism of melatonin in
indomethacin-induced gastric ulcer in a rat model. Our studies reveal that
melatonin dose-dependently downregulates the expression and secretion of
pro-MMP-9 which is induced (approximately 10-fold) during indomethacininduced
gastric ulceration. Furthermore, melatonin prevents gastric
ulceration in a dose-dependent manner through upregulation (approximately
two- to threefold) of both pro-MMP-2 and active MMP-2 at the level of
induction as well as secretion. It also prevents gastric ulcers by blocking
glutathione depletion and lipid peroxidation in cytosolic and microsomal
fractions. The novel findings of this study are attributed to the attenuation of
the pro-MMP-9 and increase of MMP-2 activity by pretreatment with
melatonin. The finding defines one of the MMP-mediated pathways for
melatonin’s action in gastric ulcer.
|
|
| Publisher |
Blackwell Publishing
|
|
| Date |
2005
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/329/1/JOURNAL_OF_PINEAL_RESEARCH_39__(3)307%2D315_2005[15].pdf
Ganguly, Krishnendu and Maity, Pallab and Reiter, Russel J and Swarnakar, Snehasikta (2005) Effect of melatonin on secreted and induced matrix metalloproteinase-9 and -2 activity during prevention of indomethacin-induced gastric ulcer. Journal Of Pineal Research, 39. pp. 307-315. |
|
| Relation |
http://www.eprints.iicb.res.in/329/
|
|