A Lupinoside prevented fatty acid induced inhibition of insulin sensitivity in 3T3 L1 adipocytes
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
A Lupinoside prevented fatty acid induced inhibition of insulin
sensitivity in 3T3 L1 adipocytes
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| Creator |
Dey, Debleena
Pal, Bikas C Biswas, Tanushree Roy, Sib Sankar Bandyopadhyay, Arun Mandal, Swapan Kumar Giri, Bir Bhanu Bhattacharya, Samir |
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| Subject |
Cell Biology & Physiology
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| Description |
The decrease in insulin sensitivity to target
tissues or insulin resistance leads to type 2 diabetes
mellitus, an insidious disease threatening global health.
Numerous evidences made free fatty acids (FFAs)
responsible for insulin resistance and type 2 diabetes.
We demonstrate here that the damage of insulin acitivity
by a free fatty acid, palmitate could be prevented
by a lupinoside. An incubation of 3T3 L1 adipocytes
with a FFA i.e. palmitate inhibited insulin stimulated
uptake of 3H-2 deoxyglucose (2 DOG) significantly.
Addition of a lupinoside purified from Pueraria tuberosa,
lupinoside PA4 (LPA4) strongly prevented this
inhibition. We then examined insulin signaling pathway
where palmitate significantly inhibited insulin
stimulated phosphorylation of Insulin receptor tyrosine
kinase, IRS 1and PI3 kinase, PDK1 and Akt/PKB.
LPA4 rescued this inhibition of signaling molecule by
palmitate. Insulin mediated translocation of Glut4, the
glucose transporter in insulin target cells, was effectively
blocked by palmitate while, LPA4 waived this
block. Administration of LPA4 to nutritionally induced
diabetic rats significantly reduced the increase in plasma glucose. All these indicate LPA4 to be a
potentially therapeutic agent for insulin resistance and
type 2 diabetes.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/342/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY%2C_300_(_1%2D2)%2C__149%2D157[81].pdf
Dey, Debleena and Pal, Bikas C and Biswas, Tanushree and Roy, Sib Sankar and Bandyopadhyay, Arun and Mandal, Swapan Kumar and Giri, Bir Bhanu and Bhattacharya, Samir (2007) A Lupinoside prevented fatty acid induced inhibition of insulin sensitivity in 3T3 L1 adipocytes. Mol Cell Biochem, 300 (1-2). pp. 149-157. |
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| Relation |
http://dx.doi.org/10.1007/s11010-006-9378-1
http://www.eprints.iicb.res.in/342/ |
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