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Hepatoprotective Activity of Liposomal Flavonoid against Arsenite-Induced Liver Fibrosis

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Hepatoprotective Activity of Liposomal Flavonoid against Arsenite-Induced Liver Fibrosis
 
Creator Mandal, Ardhendu K
Das, Subhankar
Basu, Mukul K
Chakrabarti, Rohini N
Das, Nirmalendu
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Arsenic, the environmental metalloid toxicant, is known to induce oxidative damage to liver and produce hepatic fibrosis. The theme of our study was to optimize and evaluate the therapeutic efficacy of galactosylated liposomal flavonoidal antioxidant, quercetin (QC), in combating arsenic-induced hepatic fibrogenesis. The rats of the hepatic damage group were injected s.c. a single dose of sodium arsenite (NaAsO2) (100.06 �M/kg b. wt. in 0.5 ml of physiological saline). Hepatocytes and stellate cells were separated. Mitochondrial membranes were isolated from all those separated cells. Oxidative damage was monitored at different isolated subcellular parts of different hepatic cells. Liver fibrosis was also induced by the injection of NaAsO2. Galactosylated liposomal QC injection before NaAsO2 treatment checked fibrogenesis completely by protecting the liver from oxidative attack in cellular and subcellular levels. The maximal protections from hepatocellular and fatty metamorphosis, necrosis, Kupffer cell hyperplasia, fibrosis, and in the deposition of collagen contents were observed and reconfirmed by our histopathological and histochemical analysis when rats were treated with galactosylated liposomal QC before NaAsO2 injection. Application of galactosylated liposomal QC may be a potent therapeutic approach for NaAsO2-induced fibrogenesis through a complete protection against oxidative attack in cellular and subcellular parts of rat liver.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/344/1/JOURNAL_OF_PHARMACOLOGY_AND_EXPERIMENTAL_THERAPEUTICS%2C_320(_3)%2C__994%2D1001%2C__2007[107].pdf
Mandal, Ardhendu K and Das, Subhankar and Basu, Mukul K and Chakrabarti, Rohini N and Das, Nirmalendu (2007) Hepatoprotective Activity of Liposomal Flavonoid against Arsenite-Induced Liver Fibrosis. THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS , 320 (3). pp. 994-1001.
 
Relation htpp://dx.doi.org/10.1124/jpet.106.114215.
http://www.eprints.iicb.res.in/344/