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Oxidant Induced Injury of Erythrocyte – Role of Green Tea leaf and Ascorbic Acid

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Oxidant Induced Injury of Erythrocyte – Role of Green Tea leaf and Ascorbic Acid
 
Creator Biswas, Sabyasachi
Bhattacharyya, Jharna
Dutta, Asoke G
 
Subject Cell Biology & Physiology
 
Description Oxidant and free radical-generating system were used to promote oxidative damage in erythrocytes. Among the oxidants used, phenylhydrazine represents one of the most investigated intracellular free radical-generating probes, which in the presence of haemoglobin autooxidises and give rise to hydroxyl radical, a marker for cellular damage. Erythrocyte, as a single cell, is a good model to be used for studying the haemolytic mechanism of anaemia. Our present investigations reveal increased lipid peroxidation of erythrocyte using phenylhydrazine as well as other oxygen-generating systems (hydrogen peroxide, iron with hydrogen peroxide). It has further been observed that not only lipid peroxidation, phenylhydrazine causes significant elevation in methemoglobin formation, catalase activity and turbidity, in the above system, which are the typical characteristics of haemolytic anaemia. However, exogenous administration of green tea leaf extract and ascorbic acid as natural antioxidants and free radical scavengers were shown to protect separately increased lipid peroxidation caused by phenylhydrazine, though the degree of protection is more in case of green tea leaf extract than ascorbic acid. Results suggest that oxidative damage in vivo due to haemolytic disease may be checked to some extent by using natural antioxidants.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/347/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY_276(_1%2D2)_205%2D210;2005[39].pdf
Biswas, Sabyasachi and Bhattacharyya, Jharna and Dutta, Asoke G (2005) Oxidant Induced Injury of Erythrocyte – Role of Green Tea leaf and Ascorbic Acid. Molecular and Cellular Biochemistry, 276 (1-2). pp. 205-210.
 
Relation http://dx.doi.org/10.1007/s11010-005-4062-4
http://www.eprints.iicb.res.in/347/