Interferon Gamma Promotes Survival of Lymphoblasts Overexpressing 9-O-Acetylated Sialoglycoconjugates in Childhood Acute Lymphoblastic Leukaemia (ALL)
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Interferon Gamma Promotes Survival of Lymphoblasts
Overexpressing 9-O-Acetylated Sialoglycoconjugates
in Childhood Acute Lymphoblastic Leukaemia (ALL)
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| Creator |
Ghosh, Shyamasree
Bandyopadhyay, Suman Mallick, Asish Pal, Santanu Vlasak, Reinhard Bhattacharya, Dilip K Mandal, Chitra |
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| Subject |
Infectious Diseases and Immunology
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| Description |
An enhanced linkage-specific 9-O-acetylated sialic acid (9-O-AcSA) on peripheral blood mononuclear
cells (PBMC) of children with acute lymphoblastic leukaemia, ALL (PBMCALL, 9-O-AcSAþ cells) was demonstrated by
using a lectin, Achatinin-H, whose lectinogenic epitope was 9-O-AcSAa2-6GalNAc. Our aim was to evaluate the in vitro
contributory role of this glycotope (9-O-AcSAa2-6GalNAc) towards the survival of these 9-O-AcSAþ cells in ALL patients.
For direct comparison, 9-O-AcSA� cells were generated by removing O-acetyl group of 9-O-AcSA present on PBMCALL
using O-acetyl esterase. An elevated level of serum interferon gamma (IFN-g) in affected children led us to think that
PBMCALL are continuously exposed specifically to this cytokine. Accordingly, 9-O-AcSAþ and 9-O-AcSA� cells were
exposed in vitro to IFN-g. A twofold increasedNOrelease along with inducibleNOsynthase (iNOS)mRNAexpression by
the 9-O-AcSAþ cells was observed as compared to the 9-O-AcSA� cells. The decreased viability of IFN-g exposed 9-OAcSA
� cells as compared to 9-O-AcSAþ cells were reflected from a 5.0-fold increased caspase-3-like activity and a 10.0-
fold increased apoptosis in the 9-O-AcSA� cells when production of NO was lowered by adding competitive inhibitor of
iNOS in reaction mixture. Therefore, it may be envisaged that a link exists between induction of this glycotope and their
role in regulating viability of PBMCALL. Taken together, it is reasonable to hypothesise thatO-acetylation of sialic acids on
PBMCALL may be an additional mechanism that promotes the survival of lymphoblasts by avoiding apoptosis via IFN-ginduced
NO production. J. Cell. Biochem. 95: 206–216, 2005. � 2005 Wiley-Liss, Inc.
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| Publisher |
John Wiley & Sons
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/382/1/JOURNAL_OF_CELLULAR_BIOCHEMISTRY_95(_1_)_206%2D216;2005[64].pdf
Ghosh, Shyamasree and Bandyopadhyay, Suman and Mallick, Asish and Pal, Santanu and Vlasak, Reinhard and Bhattacharya, Dilip K and Mandal, Chitra (2005) Interferon Gamma Promotes Survival of Lymphoblasts Overexpressing 9-O-Acetylated Sialoglycoconjugates in Childhood Acute Lymphoblastic Leukaemia (ALL). Journal of Cellular Biochemistry, 95 (1). pp. 206-216. ISSN 0730-2312 |
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| Relation |
http://dx.doi.org/10.1002/jcb.20382
http://www.eprints.iicb.res.in/382/ |
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