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Localization and Thyroid Hormone Influenced Expression of Collagen II in Ovarian Tissue

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Localization and Thyroid Hormone Influenced Expression of Collagen II in Ovarian Tissue
 
Creator Saha, Samir Kumar
Ghosh, Pamela
Mitra, Debanjali
Mukherjee, Satinath
Bhattacharya, Samir
Roy, Sib Sankar
 
Subject Cell Biology & Physiology
 
Description Collagen type II (Col II), one of the main components of the hyaline cartilage, is a member of the fibrilforming collagen family. Due to its amino acid composition, the extent of lysine hydroxylation of Col II is much higher than that of other fibril forming collagens. Since lysyl hydroxylase isoforms are less synthesized in hypothyroid ovarian tissue, Col II level is expected to be reduced here and contribute to the degradation of ovarian ECM in this condition. As there was no previous report, we have demonstrated Col II expression in rat ovary. Col2A1 mRNA shares significant part of the total collagens in ovary as shown by the relative expression of the major collagen genes present in this tissue. It has also been shown that Col II is down regulated in hypothyroid ovarian tissue and its expression is increased upon stimulation by thyroid hormone (T3). To know whether less Col II in hypothyroid ovarian tissue is due to less synthesis of the protein or its increased rate of degradation is also involved in it, we demonstrated the status of Collagen - degrading Matrix Metalloproteinases in this condition and found up regulation of MMP-1, -8 and - 13 in hypothyroid rat ovary. The present study shows the reduced Col II expression in hypothyroid rat ovary, with the concomitant increase in Col II degradation. This information will be useful for further studies on reproductive disorders.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/396/1/CELLULAR_PHYSIOLOGY_AND_BIOCHEMISTRY%2C_19_(_1%2D4)%2C_67%2D76%2C2007_[140].pdf
Saha, Samir Kumar and Ghosh, Pamela and Mitra, Debanjali and Mukherjee, Satinath and Bhattacharya, Samir and Roy, Sib Sankar (2007) Localization and Thyroid Hormone Influenced Expression of Collagen II in Ovarian Tissue. Cell Physiol Biochem , 19 (1-4). pp. 67-76.
 
Relation http://www.eprints.iicb.res.in/396/