Localization and Thyroid Hormone Influenced Expression of Collagen II in Ovarian Tissue
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Localization and Thyroid Hormone Influenced Expression of Collagen II in Ovarian Tissue
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Creator |
Saha, Samir Kumar
Ghosh, Pamela Mitra, Debanjali Mukherjee, Satinath Bhattacharya, Samir Roy, Sib Sankar |
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Subject |
Cell Biology & Physiology
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Description |
Collagen type II (Col II), one of the main components
of the hyaline cartilage, is a member of the fibrilforming
collagen family. Due to its amino acid
composition, the extent of lysine hydroxylation of Col
II is much higher than that of other fibril forming
collagens. Since lysyl hydroxylase isoforms are less
synthesized in hypothyroid ovarian tissue, Col II level
is expected to be reduced here and contribute to the
degradation of ovarian ECM in this condition. As there
was no previous report, we have demonstrated Col II
expression in rat ovary. Col2A1 mRNA shares
significant part of the total collagens in ovary as shown
by the relative expression of the major collagen genes
present in this tissue. It has also been shown that Col
II is down regulated in hypothyroid ovarian tissue and
its expression is increased upon stimulation by thyroid
hormone (T3). To know whether less Col II in
hypothyroid ovarian tissue is due to less synthesis of
the protein or its increased rate of degradation is also
involved in it, we demonstrated the status of Collagen - degrading Matrix Metalloproteinases in this
condition and found up regulation of MMP-1, -8 and -
13 in hypothyroid rat ovary. The present study shows
the reduced Col II expression in hypothyroid rat ovary,
with the concomitant increase in Col II degradation.
This information will be useful for further studies on
reproductive disorders.
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Date |
2007
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/396/1/CELLULAR_PHYSIOLOGY_AND_BIOCHEMISTRY%2C_19_(_1%2D4)%2C_67%2D76%2C2007_[140].pdf
Saha, Samir Kumar and Ghosh, Pamela and Mitra, Debanjali and Mukherjee, Satinath and Bhattacharya, Samir and Roy, Sib Sankar (2007) Localization and Thyroid Hormone Influenced Expression of Collagen II in Ovarian Tissue. Cell Physiol Biochem , 19 (1-4). pp. 67-76. |
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Relation |
http://www.eprints.iicb.res.in/396/
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