Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors
IR@IICB: CSIR-Indian Institute of Chemical Biology, KolkataView Archive Info
Cellobiase from Termitomyces clypeatus: activity
and secretion in presence of glycosylation inhibitors
In presence of the glycosylation
inhibitors, 2-deoxy-D-glucose (1 mg/ml), tunicamycin
(30 lg/ml), 1-deoxynojirimycin (30 lg/ml)
and D-glucono-d-lactone (1 mg/ml), total cellobiase
activity, in the extracellular, intracellular and
cell bound fractions, of the fungus Termitomyces
clypeatus grown in 20 ml cellobiose medium (1%,
w/v) increased by 50-, 1.8-, 2.4-, 1.3-fold, respectively,
with respect to control medium (16.3 U).
The inhibitors also stimulated secretion of 95% of
the total protein in culture medium, except
D-glucono-d-lactone which released 60% of the
total protein. 2-Deoxy-D-glucose (1 mg/ml) led
to production of extracellular cellobiase up to
40 U/ml, whereas in absence of the inhibitors only
0.59 U/ml enzyme was detected.
Mukherjee, Sumana and Chowdhury, Sudeshna and Ghorai, Shakuntala and Pal, Swagata and Khowala, Suman (2006) Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors. Biotechnol Lett, 28 (21). pp. 1773-1778.