Targeting of Parasite-Specific Immunoliposome- Encapsulated Doxorubicin in the Treatment of Experimental Visceral Leishmaniasis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Targeting of Parasite-Specific Immunoliposome-
Encapsulated Doxorubicin in the Treatment
of Experimental Visceral Leishmaniasis
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| Creator |
Mukherjee, Snigdha
Das, Lopamudra Kole, Labanyamoy Karmakar, Sudipan Datta, Neeta Das, Pijush K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A parasite-specific 51-kDa protein has been isolated from the membrane of macrophages infected with Leishmania
donovani, the causative agent of visceral leishmaniasis. Active targeting of doxorubicin to infected
macrophages was studied by incorporating it in immunoliposomes prepared by grafting F(ab)�2 of anti–51-
kDa antibody onto the liposomal surface. In a 45-day mouse model of visceral leishmaniasis, complete elimination
of spleen parasite burden was achieved by doxorubicin incorporated in immunoliposome (immunodoxosome)
at a dose of 250 mg/kg/day that was given for 4 consecutive days. A similar dose of free and
liposomal drug (doxosome) had 45% and 84% parasite suppressive effects, respectively. Immunodoxosome
and doxosome were generally less toxic than the free drug, as determined by several clinical parameters of
cardiotoxicity and liver toxicity. These results not only indicate the potential of doxorubicin as an effective
chemotherapeutic agent but also establish the use of immunoliposomes as drug carrier in the therapy of
leishmaniasis.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/456/1/JOURNAL_OF_INFECTIOUS_DISEASES%2C_189(_6)%2C_1024%2D1034[74].pdf
Mukherjee, Snigdha and Das, Lopamudra and Kole, Labanyamoy and Karmakar, Sudipan and Datta, Neeta and Das, Pijush K (2004) Targeting of Parasite-Specific Immunoliposome- Encapsulated Doxorubicin in the Treatment of Experimental Visceral Leishmaniasis. The Journal of Infectious Diseases , 189 (6). pp. 1024-1034. |
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| Relation |
http://dx.doi.org/10.1086/382048
http://www.eprints.iicb.res.in/456/ |
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