Transcriptional activation of tyrosinase gene by human placental sphingolipid
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Transcriptional activation of tyrosinase gene by human
placental sphingolipid
|
|
| Creator |
Saha, Bidisha
Singh, Suman Kumar Sarkar, Chinmoy Mallick, Shampa Bera, Rabindranath Bhadra, Ranjan |
|
| Subject |
Cell Biology & Physiology
|
|
| Description |
The sphingolipids, a class of complex bioactive
lipids, are involved in diverse cellular functions such
as proliferation, differentiation, and apoptosis as well as
growth inhibition. Recently sphingosylphosphorylcholine
(SPC), sphingosine-1-phosphate (S1P), and C2-ceramide
(C2-Cer), sphingolipid containing acetic acid are emerging
as melanogenic regulators. A bioactive sphingolipid (PSL)
was isolated from hydroalcoholic extract of fresh term human
placenta and it induced melanogenesis in an in vitro
culture of mouse melanoma B16F10 cells. Tyrosinase, the
rate-limiting enzyme for melanogenesis, is required to be
upregulated for the increased melanin production. The expression
of tyrosinase, both at protein as well as mRNA
level, was higher in the PSL treated B16F10 cells as evidenced
by Western blot and RT-PCR analysis. Actinomycin
D and cycloheximide, inhibitors of transcription and translation,
respectively, inhibited PSL-induced tyrosinase activity
and its protein expression showing decrease in melanogenesis,
correspondingly. The activity of GFP coupled tyrosinase
promoter was upregulated in transfected B16F10 cells after
treating with PSL as determined by fluorescence microscopy,
fluorometric analysis, and Western blot. These results, thus,
suggested that PSL upregulated tyrosinase gene expression
at transcription level through promoter activation to show
increased melanogenesis. Therefore, PSL as an inducer of
melanogenesis might account for the recovery of pigment in depigmentation disorder.
|
|
| Date |
2006
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/460/1/GLYCOCONJUGATE_JOURNAL%2C__23(_3%2D4)%2C_259%2D267_[92].pdf
Saha, Bidisha and Singh, Suman Kumar and Sarkar, Chinmoy and Mallick, Shampa and Bera, Rabindranath and Bhadra, Ranjan (2006) Transcriptional activation of tyrosinase gene by human placental sphingolipid. Glycoconj J, 23 (3-4). pp. 259-267. |
|
| Relation |
htpp://dx.doi.org/10.1007/s10719-006-7931-5
http://www.eprints.iicb.res.in/460/ |
|