Variable Degree of Alternative Complement Pathway–Mediated Hemolysis in Indian Visceral Leishmaniasis Induced by Differential Expression of 9-O–Acetylated Sialoglycans
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Variable Degree of Alternative Complement
Pathway–Mediated Hemolysis in Indian Visceral
Leishmaniasis Induced by Differential Expression
of 9-O–Acetylated Sialoglycans
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| Creator |
Chava, Anil Kumar
Chatterjee, Mitali Sharma, Vineeta Sundar, Shyam Mandal, Chitra |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Increased expression of linkage-specific 9-O–acetylated sialoglycans (9-O-AcSGs) has been demonstrated
on erythrocytes from patients with visceral leishmaniasis (VL) by use of Achatinin-H. We assessed the
capacity of this glycotope to influence hemolysis via activation of the alternative complement pathway in patients
with VL, compared with that in healthy control subjects.
Methods. The differential expression of 9-O-AcSGs on surfaces of erythrocytes was measured, 9-O-AcSGs
were affinity purified, and the molecular determinants were identified byWestern blotting. The degree of alternative
complement pathway–mediated hemolysis was compared with expression of 9-O-AcSGs on erythrocytes.
Results. Enhanced expression of linkage-specific 9-O-AcSGs was demonstrated on erythrocytes from patients
with active VL. Six distinct molecular determinants present only on diseased erythrocytes were affinity purified
and were absent after elimination of parasite burden. A correlation ( ) was observed between the presence 2 r p0.9
of 9-O-AcSGs and the degree of alternative complement pathway–mediated hemolysis.
Conclusion. The 9-O-AcSGs expressed on erythrocytes from patients with VL are potent complement activators,
causing enhanced hemolysis via activation of the alternative complement pathway, and may account for
the anemia that is a common manifestation of VL.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/461/1/JOURNAL_OF_INFECTIOUS_DISEASES%2C189(7)%2C_1257%2D1264[69].pdf
Chava, Anil Kumar and Chatterjee, Mitali and Sharma, Vineeta and Sundar, Shyam and Mandal, Chitra (2004) Variable Degree of Alternative Complement Pathway–Mediated Hemolysis in Indian Visceral Leishmaniasis Induced by Differential Expression of 9-O–Acetylated Sialoglycans. The Journal of Infectious Diseases , 189 (7). pp. 1257-1264. |
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| Relation |
http://dx.doi.org/10.1086/382752
http://www.eprints.iicb.res.in/461/ |
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