An Analytical Device for On-Site Immunoassay. Demonstration of Its Applicability in Semiquantitative Detection of Aflatoxin B1 in a Batch of Samples with Ultrahigh Sensitivity
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
An Analytical Device for On-Site Immunoassay.
Demonstration of Its Applicability in
Semiquantitative Detection of Aflatoxin B1 in a
Batch of Samples with Ultrahigh Sensitivity
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| Creator |
Pal, Arindam
Dhar, Tarun K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A simple analytical device has been developed for performing
noninstrumental immunofiltration-based assay on
a batch of samples. The device consists of membrane
strips, with antibody-immobilized zones, attached to a
polyethylene card. A moist filter paper placed between the
membrane and the polyethylene card acts as the absorbent
body. The device was used to estimate very low
concentrations of aflatoxin B1 (AFB1) present in food
samples by using an improved catalyzed reporter deposition
(Super-CARD) method of signal amplification involving
biotinylated tyramine (B-T) and avidin-horseradish
peroxidase conjugate. 4-Chloro-1-naphthol was used as
the substrate for visualization. Semiquantitative results
are obtained by visual comparison of the color intensity
(inversely related to the analyte concentration) of a sample
spot with those of reference standards. Quantitative
estimation is possible by densitometric analysis (detection
limit 0.25 pg/spot, 0.01 ng mL-1). Dilute samples can
be assayed by in situ concentration with improved doseresponse
characteristics. A batch of 12 extracted samples
can be analyzed in a single test card within 12 min. Spiked
and contaminated samples of groundnut, corn, wheat,
cheese, and chilli were analyzed without sample cleanup.
The matrix interferences were eliminated by using appropriate
dilution of the aqueous methanol extracts. Mean
recoveries from different food samples were between 91
and 104%. The values obtained for infected corn and
groundnut samples correlated well (R2 ) 0.99) with the
estimates by HPLC. The method is well-suited for visual
screening of agricultural and food samples for AFB1 under
field conditions.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/465/1/ANALYTICAL_CHEMISTRY%2C_76(_1)%2C_98%2D104[94].pdf
Pal, Arindam and Dhar, Tarun K (2004) An Analytical Device for On-Site Immunoassay. Demonstration of Its Applicability in Semiquantitative Detection of Aflatoxin B1 in a Batch of Samples with Ultrahigh Sensitivity. Analytical Chemistry, 76 (1). pp. 98-104. |
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| Relation |
http://dx.doi.org/10.1021/ac034694g
http://www.eprints.iicb.res.in/465/ |
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