Analysis of differentially expressed genes in hyperthyroid-induced hypertrophied heart by cDNA microarray
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Analysis of differentially expressed genes in hyperthyroid-induced
hypertrophied heart by cDNA microarray
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| Creator |
De, K
Ghosh, G Datta, M Konar, Aditya Bandyopadhyay, J Bandyopadhyay, D Bhattacharya, S Bandyopadhyay, A |
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| Subject |
Cell Biology & Physiology
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| Description |
Experiments were carried out to identify the altered genes
in hyperthyroid rat heart and their influence on the
functions of cardiac myocytes. Chronic treatment of rats
with 3,5,3_ triiodo-Ç¿-thyronine (T3) resulted in a prominent
increase in the size of the left ventricle with increased
wall thickness and reduced chamber volume leading to
concentric cardiac hypertrophy. The heart weight to body
weight ratio (HW/BW) in hyperthyroid rats was increased
by about 58% over that of normal rats. Using cDNA
microarray comprising 588 genes, we compared the differences
in mRNA expression of hyperthyroid and normal
rat heart. Based on a threshold of greater than 10% change,
about 37 genes were found to be regulated by T3. Further
analyses by Western blotting, Northern blotting and
real-time quantitative RT-PCR of some of the genes
confirmed the microarray results. The T3-altered genes
encode various types of proteins related to metabolism,
matrix and cytoskeletal structures, growth factors, transcription
factors, Ca2+-channels etc. The physiological
significance of one of these altered proteins in hyperthyroid
heart, insulin-responsive glucose transporter (GLUT)
type 4 (GLUT4), was studied in detail. The expression of
GLUT4 was drastically reduced in the ventricular tissues
of hyperthyroid heart. Insulin-induced glucose uptake in
hyperthyroid cardiomyocytes was reduced significantly,
indicating the impaired glucose transport in cardiac cells.
Interestingly, a few genes such as GLUT4, cytochrome
P450 isoforms, superoxide dismutase (SOD), collagens,
matrix metalloproteinases (MMP), tissue inhibitors of
matrix metalloproteinases etc. which had not been
reported earlier were found to be altered in hyperthyroid
heart. Our results show some new aspects of hyperthyroid
heart which will be important in assessing the
pathophysiology of hypertrophied cardiomyocytes.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/477/1/JOURNAL_OF_ENDOCRINOLOGY%2C_182(_2)%2C_303%2D314_[41].pdf
De, K and Ghosh, G and Datta, M and Konar, Aditya and Bandyopadhyay, J and Bandyopadhyay, D and Bhattacharya, S and Bandyopadhyay, A (2004) Analysis of differentially expressed genes in hyperthyroid-induced hypertrophied heart by cDNA microarray. Journal of Endocrinology , 182 (2). pp. 303-314. |
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| Relation |
http://dx.doi.org/10.1677/joe.0.1820303
http://www.eprints.iicb.res.in/477/ |
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