DNA Polymorphism Assay Distinguishes Isolates of Leishmania donovani That Cause Kala-Azar from Those That Cause Post-Kala-Azar Dermal Leishmaniasis in Humans
IR@IICB: CSIR-Indian Institute of Chemical Biology, KolkataView Archive Info
DNA Polymorphism Assay Distinguishes Isolates of Leishmania
donovani That Cause Kala-Azar from Those That Cause
Post-Kala-Azar Dermal Leishmaniasis in Humans
Raju, B V Subba
Nakhasi, Hira L
Structural Biology & Bioinformatics
Visceral leishmaniasis (VL), or kala-azar (KA), caused by
Leishmania donovani, is responsible for severe mortality and
morbidity with 500,000 new cases every year (2). In India the
disease is endemic in eastern parts of the country, primarily in
the state of Bihar. Post-KA dermal leishmaniasis (PKDL) is an
unusual dermatosis that develops as a sequel of KA in 10 to
20% of VL cases in India and in 60% of VL cases in Sudan (11,
19). KA transmission in India is thought to be anthroponotic,
and PKDL patients are believed to be the sole source of the
parasites (17). Studies of parasite biology in PKDL are few,
primarily because of the difficulty of culturing the parasite.
Intrinsic differences are reported to exist between the KA and
PKDL isolates (1).
One of the hallmarks of these pathogenic protozoa is the
diversity of tropism and disease resulting from infection. It is
hypothesized that reactivation of persistent infections underlies
some of the most severe forms of leishmaniasis, including
PKDL. Little is known about parasite factors required for persistent
infections (16). Moreover, humoral immune responses
in PKDL patients are distinct from those in KA patients (12).
Therefore, we sought to identify molecular differences between
parasites isolated from KA and PKDL patients that may
underlie the diversity in clinical manifestations of the disease.
The nuclear gene probe LdP13 allowed discrimination between
L. donovani and L. infantum and between geographical
isolates of L. donovani (9, 10). We utilized this probe to examine
the genetic variation in parasites isolated from Indian
KA and PKDL patients.
Sreenivas, Gannavaram and Raju, B V Subba and Singh, Ruchi and Selvapandiyan, Angamuthu and Duncan, Robert and Sarkar, Dwijen and Nakhasi, Hira L and Salotra, Poonam (2004) DNA Polymorphism Assay Distinguishes Isolates of Leishmania donovani That Cause Kala-Azar from Those That Cause Post-Kala-Azar Dermal Leishmaniasis in Humans. JOURNAL OF CLINICAL MICROBIOLOGY, 42 (4). pp. 1739-1741.