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DNA Polymorphism Assay Distinguishes Isolates of Leishmania donovani That Cause Kala-Azar from Those That Cause Post-Kala-Azar Dermal Leishmaniasis in Humans

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title DNA Polymorphism Assay Distinguishes Isolates of Leishmania donovani That Cause Kala-Azar from Those That Cause Post-Kala-Azar Dermal Leishmaniasis in Humans
 
Creator Sreenivas, Gannavaram
Raju, B V Subba
Singh, Ruchi
Selvapandiyan, Angamuthu
Duncan, Robert
Sarkar, Dwijen
Nakhasi, Hira L
Salotra, Poonam
 
Subject Structural Biology & Bioinformatics
 
Description Visceral leishmaniasis (VL), or kala-azar (KA), caused by Leishmania donovani, is responsible for severe mortality and morbidity with 500,000 new cases every year (2). In India the disease is endemic in eastern parts of the country, primarily in the state of Bihar. Post-KA dermal leishmaniasis (PKDL) is an unusual dermatosis that develops as a sequel of KA in 10 to 20% of VL cases in India and in 60% of VL cases in Sudan (11, 19). KA transmission in India is thought to be anthroponotic, and PKDL patients are believed to be the sole source of the parasites (17). Studies of parasite biology in PKDL are few, primarily because of the difficulty of culturing the parasite. Intrinsic differences are reported to exist between the KA and PKDL isolates (1). One of the hallmarks of these pathogenic protozoa is the diversity of tropism and disease resulting from infection. It is hypothesized that reactivation of persistent infections underlies some of the most severe forms of leishmaniasis, including PKDL. Little is known about parasite factors required for persistent infections (16). Moreover, humoral immune responses in PKDL patients are distinct from those in KA patients (12). Therefore, we sought to identify molecular differences between parasites isolated from KA and PKDL patients that may underlie the diversity in clinical manifestations of the disease. The nuclear gene probe LdP13 allowed discrimination between L. donovani and L. infantum and between geographical isolates of L. donovani (9, 10). We utilized this probe to examine the genetic variation in parasites isolated from Indian KA and PKDL patients.
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/487/1/JOURNAL_OF_CLINICAL_MICROBIOLOGY%2C_42(_4)%2C_1739%2D1741[66].pdf
Sreenivas, Gannavaram and Raju, B V Subba and Singh, Ruchi and Selvapandiyan, Angamuthu and Duncan, Robert and Sarkar, Dwijen and Nakhasi, Hira L and Salotra, Poonam (2004) DNA Polymorphism Assay Distinguishes Isolates of Leishmania donovani That Cause Kala-Azar from Those That Cause Post-Kala-Azar Dermal Leishmaniasis in Humans. JOURNAL OF CLINICAL MICROBIOLOGY, 42 (4). pp. 1739-1741.
 
Relation http://dx.doi.org/10.1128/JCM.42.4.1739-1741.2004
http://www.eprints.iicb.res.in/487/