Human placental lipid induces melanogenesis through p38 MAPK in B16F10 mouse melanoma
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Human placental lipid induces melanogenesis through
p38 MAPK in B16F10 mouse melanoma
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| Creator |
Singh, Suman Kumar
Sarkar, Chinmoy Mallick, Shampa Saha, Bidisha Bera, Rabindranath Bhadra, Ranjan |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Melanogenesis is one of the characteristic functional
activities of melanocyte/melanoma and is
regulated via mitogen-activated protein kinase
(MAPK) and Akt/protein kinase B (PKB) pathways.
Placental total lipid fraction (PTLF), prepared from
a hydroalcoholic extract of fresh term human placenta
contains sphingolipids and was recently
shown to stimulate melanogenesis via up-regulation
of the key enzyme tyrosinase in B16F10
mouse melanoma cells. How such lipids mediate
their effects on pigmentation and tyrosinase
expression is a particularly important aspect of
melanogenesis. To study the signaling that leads
to tyrosinase expression, we have investigated the
roles of the MAPK and Akt/PKB pathways in
B16F10 melanoma cells in melanogenesis in
response to PTLF. Treatment of cells with PTLF led
to the time dependent phosphorylation of p38
MAPK. SB203580, a p38 MAPK inhibitor, completely
blocked the PTLF-induced melanogenesis by
inhibiting promoter activity and subsequent
expression of tyrosinase. Phosphatidylinositol 3-
kinase (PI3K) inhibitor, LY294002 a blocker of the
Akt signaling pathway, or an inhibitor of MEK
(MAPK/ERK Kinase), PD98059 when included along
with PTLF was found to potentiate PTLF-induced
phosphorylation of p38 MAPK together with tyrosinase
expression and melanogenesis. The results
suggest that the activation of p38 MAPK plays a
crucial role in PTLF-induced B16F10 melanogenesis
by up-regulating tyrosinase expression.
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| Publisher |
Blackwell Publishing
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/517/1/PIGMENT_CELL_RESEARCH_18_(_2_)113%2D121;2005[79].pdf
Singh, Suman Kumar and Sarkar, Chinmoy and Mallick, Shampa and Saha, Bidisha and Bera, Rabindranath and Bhadra, Ranjan (2005) Human placental lipid induces melanogenesis through p38 MAPK in B16F10 mouse melanoma. Pigment Cell Research, 18 (2). pp. 113-121. |
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| Relation |
http://dx.doi.org/10.1111/j.1600-0749.2005.00219
http://www.eprints.iicb.res.in/517/ |
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