Primordial Germ Cell Migration in the Rat: Preliminary Evidence for a Role of Galactosyltransferase1
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Primordial Germ Cell Migration in the Rat: Preliminary Evidence for a Role
of Galactosyltransferase1
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| Creator |
Bandyopadhyay, Soma
Banerjee, Sutapa Pal, Alok K Goswami, S K Chakravarty, B N Kabir, S N |
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| Subject |
Cell Biology & Physiology
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| Description |
The precise cellular mechanism of primordial germ cell
(PGC) migration remains unknown. Cell surface galactosyltransferase
(GalTase) is known to play unique roles in the process of
locomotion of many migratory cells. With an objective to seek
evidence for possible involvement of GalTase in the migratory
process of PGC, we evaluated germ cell migration in the rat
following experimental modulation of embryonic GalTase activity.
Pregnant rats were laparotomized under anesthesia on Day
10 of pregnancy. While embryos of one uterine horn received
lysozyme (100 mg/fetus), those of the other received a-lactalbumin
(LA; 100 mg/fetus), N-acetylglucosamine (GlcNAc; 250
nmole/fetus), uridine 59-monophosphate (UMP; 2.5 mmole/fetus),
uridine diphosphate-galactose (UDP-gal; 250 nmole/fetus),
or a combination of 250 nmole of UDP-gal and 2.5 mmole of
UMP/fetus. Between gestation Days 12 and 14, embryos were
dissected out and processed for histochemical localization of
PGC on the basis of binding of Dolichos biflorus agglutinin on
the surface glycoconjugate of the germ cells. The number of
PGC in each embryo was counted. There was a daywise increase
in the number of PGC in all groups. As compared with lysozymeexposed
controls, the numbers of PGCs at the day-specific sites
on all days of examination were significantly lower in the LAas
well as GlcNAc-exposed groups. UMP or UDP-gal individually
exerted little or no influence, while the total PGC count
rose significantly over the respective control values under simultaneous
exposure to UMP and UDP-gal. The present findings
suggest a likely catalytic role of GalTase in the process of germ
cell migration.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/519/1/BIOLOGY_OF_REPRODUCTION_%2C_71(_6)%2C__1822%2D1827_[3].pdf
Bandyopadhyay, Soma and Banerjee, Sutapa and Pal, Alok K and Goswami, S K and Chakravarty, B N and Kabir, S N (2004) Primordial Germ Cell Migration in the Rat: Preliminary Evidence for a Role of Galactosyltransferase1. Biology of Reproduction, 71 (6). pp. 1822-1827. |
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| Relation |
http://dx.doi.org/10.1095/biolreprod.104.028555
http://www.eprints.iicb.res.in/519/ |
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