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Effect of Bile on the Cell Surface Permeability Barrier and Efflux System of Vibrio cholerae

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Effect of Bile on the Cell Surface Permeability Barrier and Efflux System of Vibrio cholerae
 
Creator Chatterjee, Arpita
Chaudhuri, Sohini
Saha, Gargi
Gupta, Satadeepa
Chowdhury, Rukhsana
 
Subject Infectious Diseases and Immunology
 
Description Gram-negative bacteria are inherently impermeable to hydrophobic compounds, due to the synergistic activity of the permeability barrier imposed by the outer membrane and energy dependent efflux systems. The gram-negative, enteric pathogen Vibrio cholerae appears to be deficient in both these activities; the outer membrane is not an effective barrier to hydrophobic permeants, presumably due to the presence of exposed phospholipids on the outer leaflet of the outer membrane, and efflux systems are at best only partially active. When V. cholerae was grown in the presence of bile, entry of hydrophobic compounds into the cells was significantly reduced. No difference was detected in the extent of exposed phospholipids on the outer leaflet of the outer membrane between cells grown in the presence or absence of bile. However, in the presence of energy uncouplers, uptake of hydrophobic probes was comparable between cells grown in the presence or absence of bile, indicating that energy-dependent efflux processes may be involved in restricting the entry of hydrophobic permeants into bile grown cells. Indeed, an efflux system(s) is essential for survival of V. cholerae in the presence of bile. Expression of acrAB, encoding an RND family efflux pump, was significantly increased in V. cholerae cells grown in vitro in the presence of bile and also in cells grown in rabbit intestine.
 
Publisher American Society for Microbiology
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/539/1/JOURNAL_OF_BACTERIOLOGY%2C_186(_20)%2C_6809%2D6814_[16].pdf
Chatterjee, Arpita and Chaudhuri, Sohini and Saha, Gargi and Gupta, Satadeepa and Chowdhury, Rukhsana (2004) Effect of Bile on the Cell Surface Permeability Barrier and Efflux System of Vibrio cholerae. Journal of Bacteriology, 186 (20). pp. 6809-6814.
 
Relation http://dx.doi.org/10.1128/JB.186.20.6809–6814.2004
http://www.eprints.iicb.res.in/539/