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Characterization of Leishmania donovani Antigens Encapsulated in Liposomes That Induce Protective Immunity in BALB/c Mice

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Characterization of Leishmania donovani Antigens Encapsulated in Liposomes That Induce Protective Immunity in BALB/c Mice
 
Creator Afrin, Farhat
Rajesh, Ravindran
Anam, Khairul
Gopinath, Meenakshisundram
Pal, Swati
Ali, Nahid
 
Subject Infectious Diseases and Immunology
 
Description Leishmania donovani promastigote membrane antigens (LAg) encapsulated in positively charged liposomes have been found to induce very significant levels of protection against experimental visceral leishmaniasis. The protectively immunized animals exhibited profound delayed-type hypersensitivity and antibody responses. The extent of protection induced by the same antigens, however, varied depending on the charge of the vesicles, with maximum induction by positively charged liposomes, followed by neutral liposomes and last negatively charged liposomes. Characterization of LAg and LAg entrapped in liposomes of different charges by Western blot analysis revealed the immunodominance of gp63 in all three vaccine preparations. The strong reactivity of antigens in a restricted antigen profile that included, in addition to gp63, 72-, 52-, 48-, 45-, 39-, and 20-kDa components in neutral and positively charged liposomes contrasted with the reactivity of a greater number of LAg components in negatively charged liposomes. Resistance to visceral leishmaniasis appears to depend on the immunity induced by gp63 and a few select antigens in association with the right liposomes. A striking similarity between the immunogenic profile of partially purified soluble antigens and that of LAg in neutral and positively charged liposomes suggests the potentiality of these antigens in future vaccine studies of L. donovani.
 
Publisher American Society for Microbiology
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/541/1/INFECTION_AND_IMMUNITY_70__(12)6697%2D6706_;2002[6].pdf
Afrin, Farhat and Rajesh, Ravindran and Anam, Khairul and Gopinath, Meenakshisundram and Pal, Swati and Ali, Nahid (2002) Characterization of Leishmania donovani Antigens Encapsulated in Liposomes That Induce Protective Immunity in BALB/c Mice. Infection and Immunity, 70 (12). pp. 6697-6706.
 
Relation http://dx.doi.org/10.1128/IAI.70.12.6697–6706.2002
http://www.eprints.iicb.res.in/541/