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Monoclonal antibody affinity purification of a 78 kDa membrane protein of Leishmania donovani of Indian origin and its role in host–parasite interaction

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Monoclonal antibody affinity purification of a 78 kDa membrane protein of Leishmania donovani of Indian origin and its role in host–parasite interaction
 
Creator Mukherjee, Mandira
Bhattacharyya, Anindita
Duttagupta, Swadesh
 
Subject Structural Biology & Bioinformatics
 
Description Monoclonal antibodies were raised against pathogenic promastigotes of Leishmania donovani of Indian origin. Among these, one was used for immuno-affinity purification of a 78 kDa membrane protein present in both the amastigote and promastigote forms of the parasite. Results of immunoblot experiments with the anti-78 kDa antibody revealed that the protein was present only in parasites belonging to the L. donovani complex. The expression of the protein was observed to be the same during different phases of growth of the promastigotes. Therefore, the 78 kDa protein is neither stage-specific nor differentially regulated. Surface iodination and subcellular fractionation of the promastigotes indicated that the protein was localized on the cell surface. The 78 kDa protein was found to inhibit the binding of promastigotes to macrophages significantly, suggesting that it may play a role in the process of infection. Thus, here we report the purification of a surface protein of L. donovani of Indian origin, which may play an important role in the process of infection.
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/545/1/JOURNAL_OF_BIOSCIENCES_27(7_)_665%2D672;2002[10].pdf
Mukherjee, Mandira and Bhattacharyya, Anindita and Duttagupta, Swadesh (2002) Monoclonal antibody affinity purification of a 78 kDa membrane protein of Leishmania donovani of Indian origin and its role in host–parasite interaction. Journal of Biosciences, 27 (7). pp. 665-672.
 
Relation http://dx.doi.org/10.1007/BF02708374
http://www.eprints.iicb.res.in/545/