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Isolation, characterization and expression of a cyclin from Leishmania donovani

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Isolation, characterization and expression of a cyclin from Leishmania donovani
 
Creator Banerjee, Sampali
Banerjee, Rahul
Das, Ruma
Duttagupta, Swadesh
Saha, Partha
 
Subject Molecular & Human Genetics
 
Description We have cloned and sequenced a DNA fragment (V1 kb) containing a complete open reading frame from a cDNA library of Leishmania donovani promastigotes. The alignment of the derived polypeptide sequence and the modeling studies revealed that the protein is highly homologous to the mammalian cyclins having conserved cyclin box and substrate-docking motif. Northern blot analysis of the RNA isolated from synchronized L. donovani promastigotes showed periodic expression of the message with maximum abundance at S-phase suggesting its involvement in the events related to the regulation of DNA replication. The results confirm that we have isolated a cyclin molecule from L. donovani (LdCyc1) which may play an important role in the regulation of the parasite cell cycle. 5 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/547/1/FEMS_MICROBIOLOGY_LETTERS%2C226(_2)%2C_285%2D289[34].pdf
Banerjee, Sampali and Banerjee, Rahul and Das, Ruma and Duttagupta, Swadesh and Saha, Partha (2003) Isolation, characterization and expression of a cyclin from Leishmania donovani. FEMS Microbiology Letters , 226 (2). pp. 285-289.
 
Relation http://dx.doi.org/10.1016/S0378-1097(03)00606-2
http://www.eprints.iicb.res.in/547/