Detection of Immune-Complexed 9-O-Acetylated Sialoglycoconjugates in the Sera of Patients with Pediatric Acute Lymphoblastic Leukemia
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Detection of Immune-Complexed 9-O-Acetylated Sialoglycoconjugates in the Sera of Patients with Pediatric
Acute Lymphoblastic Leukemia
|
|
| Creator |
Bandyopadhyay, Suman
Mukherjee, Kankana Chatterjee, Mitali Bhattacharya, D K Mandal, Chitra |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
Although childhood acute lymphoblastic leukemia (ALL) is highly responsive to chemotherapy, reliable techniques
are needed to determine treatment outcome. Over expression of 9-O-acetylated sialoglycoconjugates (9-OAcSGs) on
lymphoblasts and concomitant anti-9-OAcSGs was found to have a diagnostic and prognostic potential. However, the
presence of circulatory immune-complexed antigens remains unknown. The present study was aimed to evaluate
whether immune-complexed 9-OAcSGs can be harnessed for better disease management. Immune-complexed antigens
were evaluated in ALL sera (n=262) by a Dot-blot using a 9-OAcSAa2-6GalNAc-specific lectin, Achatinin-H. Using
three serum samples, the inter- and intra-assay imprecision was evaluated as 11–13% and 7–11%, respectively. The
recovery of spiked 9-OAcSGs was 84.2–95.4%. The central 95% reference interval for immune-complexed 9-OAcSGs
in normal human sera (NHS, n=144) was 2.9–3.4 Ag/ml irrespective of sex and age. At disease presentation, the
immune-complexed 9-OAcSGs were fivefold higher than NHS, decreased with remission induction and importantly,
reappeared with clinical relapse. Sera from patients with other hematological disorders (n=86) showed negligible levels.
The Dot-blot demonstrated the potential application of immune-complexed antigen as a disease-specific marker and its
|
|
| Date |
2005
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/552/1/JOURNAL_OF_IMMUNOLOGICAL_METHODS__297_(1%2D2)_13%2D;2005[98].pdf
Bandyopadhyay, Suman and Mukherjee, Kankana and Chatterjee, Mitali and Bhattacharya, D K and Mandal, Chitra (2005) Detection of Immune-Complexed 9-O-Acetylated Sialoglycoconjugates in the Sera of Patients with Pediatric Acute Lymphoblastic Leukemia. Journal of Immunological Methods, 297 (1-2). pp. 13-26. |
|
| Relation |
http://dx.doi.org/10.1016/j.jim.2004.11.014
http://www.eprints.iicb.res.in/552/ |
|