Genesis of variants of Vibrio cholerae O1 biotype El Tor: role of the CTXf array and its position in the genome
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Genesis of variants of Vibrio cholerae O1 biotype
El Tor: role of the CTXf array and its position in
the genome
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| Creator |
Nandi, Suvobroto
Maiti, Diganta Saha, Arjun Bhadra, Rupak K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
The gene encoding cholera toxin, the principal virulence factor of Vibrio cholerae, is encoded by a
filamentous, lysogenic bacteriophage known as CTXf. The genome of V. cholerae, the host for
CTXf, consists of two chromosomes, one large and one small. Here, it is shown that localization
and array of CTX prophage DNA in either the large or small chromosome of V. cholerae is likely to be
one of the reasons for the emergence of O1 biotype El Tor variants isolated just before and after the
V. cholerae O139 cholera outbreak in 1992. Analyses of the organization of the CTX region of the
genome of pre-O139 El Tor strains revealed that these strains carry two distinct CTX prophages
integrated in the small chromosome in tandem: CTXET, the prophage having a conserved NotI site in
its repeat sequence segment which seems to be specific for the El Tor strains so far examined,
followed by CTXcalc-like genome, the prophage found in recent O139 clinical isolates from
Calcutta. In sharp contrast, in post-O139 El Tor strains only one copy of the CTXET prophage was
found to be integrated in the large chromosome. To the authors’ knowledge, the presence of CTX
prophage in the small chromosome of O1 El Tor strains has not been reported previously.
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| Publisher |
American Society for Microbiology
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| Date |
2003
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/565/1/MICROBIOLOGY%2DSGM%2C149%2C_89%2D97[95].pdf
Nandi, Suvobroto and Maiti, Diganta and Saha, Arjun and Bhadra, Rupak K (2003) Genesis of variants of Vibrio cholerae O1 biotype El Tor: role of the CTXf array and its position in the genome. Journal of Clinical Microbiology, 149. pp. 89-97. |
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| Relation |
http://dx.doi.org/10.1099/mic.0.25599-0
http://www.eprints.iicb.res.in/565/ |
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