Identification of the gene for the monomeric alkaline phosphatase of Vibrio cholerae serogroup O1 strain
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Identification of the gene for the monomeric alkaline phosphatase
of Vibrio cholerae serogroup O1 strain
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| Creator |
Majumdar, Anasuya
Ghatak, Atreyi Ghosh, Ranajit K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Alkaline phosphatase (APase) of Vibrio cholerae is the first monomeric alkaline phosphatase reported [Roy, N.K., Ghosh, R.K., Das, J.,
1982a. Monomeric alkaline phosphatase of V. cholerae. J. Bacteriol. 150, 1033–1039.]. The gene (phoAVC) encoding this enzyme is not
identified in the published genome sequence of the V. cholerae serogroup O1 El Tor strain N16961 [Heidelberg et al., 2000, DNA sequence
of both the chromosome of cholera pathogen V. cholerae. Nature 406, 477–484.]. However two genes (phoBVC and phoRVC) regulating the
synthesis of alkaline phosphatase in this organism, equivalent to phoB and phoR of Escherichia coli, are located in tandem on chromosome I
of V. cholerae. We have identified the phoAVC gene on the N16961 genome sequence by amino acid sequence analysis of the purified
alkaline phosphatase of V. cholerae classical strain 569B followed by BLAST search. The gene was found to be located on the hypothetical
protein locus VCA0033 of chromosome II. The identity of the gene was confirmed by expressing the cloned VCA0033 locus in phoA
mutant E. coli E15 and JC9223 cells and isolating V. cholerae monomeric alkaline phosphatase. Insertional inactivation of the gene also
resulted in complete loss of the phenotype. Unlike in E. coli where phoB, phoR and phoA are closely linked, phoAVC is not linked to
phoBVC and phoRVC.
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/571/1/GENE___344_%2C251%2D258_;2005[104].pdf
Majumdar, Anasuya and Ghatak, Atreyi and Ghosh, Ranajit K (2005) Identification of the gene for the monomeric alkaline phosphatase of Vibrio cholerae serogroup O1 strain. Gene, 344. pp. 251-258. |
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| Relation |
http://dx.doi.org/10.1016/j.gene.2004.11.005
http://www.eprints.iicb.res.in/571/ |
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