Nucleic Acid Sequence Analysis of Basal Core Promoter/Precore/Core Region of Hepatitis B Virus Isolated from Chronic Carriers of the Virus from Kolkata, Eastern India: Low Frequency of Mutation in the Precore Region
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Nucleic Acid Sequence Analysis of Basal Core Promoter/Precore/Core Region of Hepatitis B
Virus Isolated from Chronic Carriers of the Virus
from Kolkata, Eastern India: Low Frequency of
Mutation in the Precore Region
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| Creator |
Banerjee, Arup
Banerjee, Soma Chowdhury, Abhijit Santra, Amal Chowdhury, Sujit Roychoudhury, Susanta Panda, Chinmoy Kumar Bhattacharya, Sujit Kumar |
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| Subject |
Molecular & Human Genetics
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| Description |
The aim of the present study was to characterize
the predominant hepatitis B virus (HBV) strains and
their molecular variants present in the HBV isolates of
the different genotypes found among the chronic carriers
of the virus in our community. Methods: Precore/core
and core promoter regions of HBV DNA were amplifi ed
by polymerase chain reaction and then subjected to direct
sequencing. Of the 64 hepatitis B surface antigen
(HBsAg)-positive chronic HBV carriers investigated, 44
were HBeAg negative and 20 were HBeAg positive. Results:
In addition to genotype D, which was the predominant
ge notype, 12 genotype C (18.7%) and 6 genotype
A (9.4%) were also detected. Presence of T at nt 1858 has
often been related to the development of precore stop
mutation at nt 1896, while that of C has been related to
the development of 1762–1764 double mutation. In our
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/573/1/INTERVIROLOGY_48_(_6)_389%2D399_;2005[108].pdf
Banerjee, Arup and Banerjee, Soma and Chowdhury, Abhijit and Santra, Amal and Chowdhury, Sujit and Roychoudhury, Susanta and Panda, Chinmoy Kumar and Bhattacharya, Sujit Kumar (2005) Nucleic Acid Sequence Analysis of Basal Core Promoter/Precore/Core Region of Hepatitis B Virus Isolated from Chronic Carriers of the Virus from Kolkata, Eastern India: Low Frequency of Mutation in the Precore Region. Intervirology, 48 (6). pp. 389-399. |
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| Relation |
http://dx.doi.org/10.1159/000086066
http://www.eprints.iicb.res.in/573/ |
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