Human placental lipid induces melanogenesis by increasing the expression of tyrosinase and its related proteins in vitro
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Human placental lipid induces melanogenesis by
increasing the expression of tyrosinase and its related
proteins in vitro
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| Creator |
Mallick, Shampa
Singh, Suman Kumar Sarkar, Chinmoy Saha, Bidisha Bhadra, Ranjan |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Lipids, particularly sphingolipids, are emerging as
novel regulators of cellular activity. A placental total
lipid fraction (PTLF), the total lipid prepared from an
hydroalcoholic extract of fresh term human
placenta, was previously shown to have a pigmentinducing
activity in an animal model. The PTLF contains
sphingolipids which stimulate DNA synthesis
and melanin formation with marked morphological
changes in B16F10 melanoma cells. In order to identify
the mechanism underlying the increased melanin
synthesis, B16F10 cells were treated with PTLF
to assess the catalytic activities of tyrosinase (i.e.
tyrosine hydroxylase and DOPA oxidase), the key
regulatory enzyme of melanin synthesis. Tyrosine
hydroxylase (estimated by the release of 3H2O) as
well as DOPA oxidase (measured spectrophotometrically
and also in non-denaturing gels), was stimulated
significantly by PTLF. Western blot analysis
demonstrated an increase in the expression of
tyrosinase, tyrosinase related proteins 1 and 2
(TRP1 and TRP2) at the protein level and RT-PCR
analysis revealed stimulated transcription of tyrosinase,
TRP1 and TRP2 mRNAs in PTLF-treated B16F10
cells. Actinomycin D and cycloheximide, inhibitors
of transcription and translation, respectively, inhibited
PTLF-induction of tyrosinase activity with a corresponding
decrease in melanogenesis. In all cases,
the response to PTLF was similar to that induced by
a-melanocyte stimulating hormone, a well-known
stimulator of melanogenesis. Thus, these results
provide the basis of action of PTLF stimulated
melanogenesis in B16F10 cells showing that this placental extract is a strong inducer of pigmentation
at the transcriptional and translational levels.
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| Publisher |
Blackwell Publishing
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/583/1/PIGMENT_CELL_RESEARCH__18_(1)_25%2D33;2005[99].pdf
Mallick, Shampa and Singh, Suman Kumar and Sarkar, Chinmoy and Saha, Bidisha and Bhadra, Ranjan (2005) Human placental lipid induces melanogenesis by increasing the expression of tyrosinase and its related proteins in vitro. Pigment Cell Research, 18 (1). pp. 25-33. |
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| Relation |
http://dx.doi.org/10.1111/j.1600-0749.2004.00193
http://www.eprints.iicb.res.in/583/ |
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