Prenatal exposure to high galactose adversely affects initial gonadal pool of germ cells in rats
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Prenatal exposure to high galactose adversely affects initial
gonadal pool of germ cells in rats
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| Creator |
Bandyopadhyay, Santu
Chakrabarti, J Banerjee, S Pal, A K Bhattacharyya, D Goswami, S K Chakravarty, B N Kabir, S N |
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| Subject |
Cell Biology & Physiology
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| Description |
In rats, prenatal exposure to high concentrations of galactose may contribute to a condition that
is equivalent to the premature ovarian failure (POF) component of human galactosaemia. We investigated if development
of POF under experimental galactosaemia-like conditions was attributed to impaired germ cell migration.
Pregnant rats were fed pellets supplemented with, or without, 35% galactose from day 3 of conception
continuing through parturition. Between days 12±15, embryos from one uterine horn were dissected out. Primordial
germ cells (PGC) were histochemically localized and counted on the basis of binding of Dolichos bi¯orus agglutinin,
a lectin speci®c for terminal N-acetylgalactosamine (GalNAc), to the surface glycoconjugate of the germ cells. The
embryos from the other uterine horn were maintained until parturition. Liver activity of uridine diphosphate galactose
4-epimerase, the enzyme involved at multiple steps in the process of synthesis of GalNAc, was assayed in 1±2
day old female pups.The numbers of PGC at the day-speci®c sites on all days of examination were signi
®cantly lower (P < 0.0003), and liver epimerase activity was signi®cantly (P = 0.000001) reduced in the galactoseexposed
group.Impaired germ cell migration leading to the development of gonads with de®cient
initial pools of germ cells may form the causal link between galactosaemia and POF.
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| Publisher |
Oxford University Press
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| Date |
2003
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/604/1/HUMAN_REPRODUCTION%2C_18_(2)%2C_276%2D282[83].pdf
Bandyopadhyay, Santu and Chakrabarti, J and Banerjee, S and Pal, A K and Bhattacharyya, D and Goswami, S K and Chakravarty, B N and Kabir, S N (2003) Prenatal exposure to high galactose adversely affects initial gonadal pool of germ cells in rats. Human Reproduction, 18 (2). pp. 276-282. |
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| Relation |
http://dx.doi.org/10.1093/humrep/deg058
http://www.eprints.iicb.res.in/604/ |
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