Structural and functional studies of eukaryotic topoisomerase I with special reference to Leishmania donovani type I DNA topoisomerase
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Structural and functional studies of eukaryotic
topoisomerase I with special reference to
Leishmania donovani type I DNA topoisomerase
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| Creator |
Ganguly, Agneyo
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| Subject |
Infectious Diseases and Immunology
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| Description |
Soon after the double-helical structure was proposed for DNA (Watson and Crick, 1953a), the replication of the circular DNA molecule of a prokaryote raised an intriguing question: How did the two sister double-stranded DNA (dsDNA) molecules disentangle (Watson and Crick, 1953b)? This mechanical problem of separating two intertwined chains became a topological one. In such a
molecule, the two single stranded rings are topologically linked, and therefore cannot come apart without at least one transient break in one of the two strands. The order or extent of topological linkage of the two strands is expressed by their linking number (Lk). Lk is defined as the number of times the two single strands revolve around each other. A right handed double helix with n base pairs will make one full turn every 10.5 base pairs under physiological conditions in its
most stable structure. Therefore, its linking number (Lk0) is expected to be n / 10.5; the superscript specifying the most stable state of the molecule
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| Date |
2008
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| Type |
Thesis
NonPeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/607/1/AGNEYO_THESIS.pdf
Ganguly, Agneyo (2008) Structural and functional studies of eukaryotic topoisomerase I with special reference to Leishmania donovani type I DNA topoisomerase. PhD thesis, Jadavpur University. |
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| Relation |
http://www.eprints.iicb.res.in/607/
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