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Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte
 
Creator Dasgupta, S
Basu, D
Kumar, L Ravi
Bhattacharya, S
 
Subject Chemistry
 
Description Meiotic arrest of oocyte in an Indian carp, Labeo rohita Ham. has been found for the first time to be withdrawn by insulin only. Addition of insulin to oocytes in vitro caused germinal vesicle breakdown (GVBD), one of the first visual markers to determine initiation of the final maturational process. Under the influence of insulin the germinal vesicle (GV) of the oocyte migrated towards the animal pole, reached the micropyle and then dissolved (GVBD). By using different concentrations of insulin i.e., 0×063, 0×63, 6×3 and 12×6 mM, optimum amount required was found to be 6×3 mM. Induction of GVBD by insulin could be blocked by cycloheximide (Chx), a translation inhibitor, while actinomycin D (AcD) had no effect suggesting non-involvement of transcriptional activity in this process. Addition of the maturation-inducing steroid 17a,20b-dihydroxy-4-pregnen-3-one (DHP) stimulated (P < 0×01) GVBD of carp oocytes and its combination with insulin showed an additive effect. Gonadotropin (GtH) caused GVBD but its effect was greatly augmented by insulin. Our results demonstrate that not only can insulin alone induce GVBD in carp oocytes, but it also augments the stimulatory effect of DHP or IGF-I or GtH on GVBD. This information will be important in hormonal manipulation during induced breeding of carp.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/619/1/JOURNAL_OF_BIOSCIENCES%2C26(_3)%2C_341%2D347%2C2001[22].pdf
Dasgupta, S and Basu, D and Kumar, L Ravi and Bhattacharya, S (2001) Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte. Journal Of Bioscience, 26 (3). pp. 341-347.
 
Relation http://dx.doi.org/10.1007/BF02703743
http://www.eprints.iicb.res.in/619/