Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte
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Creator |
Dasgupta, S
Basu, D Kumar, L Ravi Bhattacharya, S |
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Subject |
Chemistry
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Description |
Meiotic arrest of oocyte in an Indian carp, Labeo rohita Ham. has been found for the first time to be withdrawn
by insulin only. Addition of insulin to oocytes in vitro caused germinal vesicle breakdown (GVBD), one of the
first visual markers to determine initiation of the final maturational process. Under the influence of insulin the
germinal vesicle (GV) of the oocyte migrated towards the animal pole, reached the micropyle and then dissolved
(GVBD). By using different concentrations of insulin i.e., 0×063, 0×63, 6×3 and 12×6 mM, optimum amount
required was found to be 6×3 mM. Induction of GVBD by insulin could be blocked by cycloheximide (Chx), a
translation inhibitor, while actinomycin D (AcD) had no effect suggesting non-involvement of transcriptional
activity in this process. Addition of the maturation-inducing steroid 17a,20b-dihydroxy-4-pregnen-3-one (DHP)
stimulated (P < 0×01) GVBD of carp oocytes and its combination with insulin showed an additive effect. Gonadotropin
(GtH) caused GVBD but its effect was greatly augmented by insulin. Our results demonstrate that
not only can insulin alone induce GVBD in carp oocytes, but it also augments the stimulatory effect of DHP
or IGF-I or GtH on GVBD. This information will be important in hormonal manipulation during induced
breeding of carp.
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Date |
2001
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/619/1/JOURNAL_OF_BIOSCIENCES%2C26(_3)%2C_341%2D347%2C2001[22].pdf
Dasgupta, S and Basu, D and Kumar, L Ravi and Bhattacharya, S (2001) Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte. Journal Of Bioscience, 26 (3). pp. 341-347. |
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Relation |
http://dx.doi.org/10.1007/BF02703743
http://www.eprints.iicb.res.in/619/ |
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