Stepwise Transfer of tRNA through the Double Membrane of Leishmania Mitochondria
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Stepwise Transfer of tRNA through the Double Membrane of
Leishmania Mitochondria
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| Creator |
Mukherjee, Shankar
Bhattacharyya, Suvendra Nath Adhya, Samit |
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| Subject |
Molecular & Human Genetics
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| Description |
Import of tRNA into Leishmania mitochondria involves
transfer through a double membrane barrier. To
examine whether specific sorting mechanisms for individual
tRNAs direct them to different mitochondrial
compartments, the distribution of tRNA transcripts, internalized
in vitro, was examined by suborganellar fractionation.
Significant amounts of tRNATyr were localized
in the matrix and on the outer face of the inner
mitochondrial membrane. With time, the matrix:membrane
ratio increased. Translocation through the inner
membrane apparently required the presence of a specific
signal in the D arm of tRNATyr, and tRNAGln(CUG),
lacking this sequence, was excluded. Hydrolysis of ATP
was necessary at both the outer and inner membranes.
However, the protonophores carbonylcyanide m-chlorophenylhydrazone
and nigericin, the K1 ionophore valinomycin,
and the F1F0 ATPase inhibitor oligomycin had
only marginal effects on uptake through the outer membrane
but severely inhibited inner membrane translocation,
indicating the unusual requirement of both the
electrical and chemical components of the electromotive
force generated across the inner membrane. The
results are consistent with a mechanism involving stepwise
transfer of tRNA through distinct outer and inner
membrane channels.
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| Publisher |
American Society for Biochemistry and Molecular Biology
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/677/1/JOURNAL_OF_BACTERIOLOGY__181(_3)_879%2D883;1999[78].pdf
Mukherjee, Shankar and Bhattacharyya, Suvendra Nath and Adhya, Samit (1999) Stepwise Transfer of tRNA through the Double Membrane of Leishmania Mitochondria. The Journal of Biological Chemistry, 274 (44). pp. 31249-31255. |
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| Relation |
http://dx.doi.org/10.1074/jbc.274.44.31249
http://www.eprints.iicb.res.in/677/ |
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