The mutK Gene of Vibrio cholerae: a New Gene Involved in DNA Mismatch Repair
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
The mutK Gene of Vibrio cholerae: a New Gene Involved in
DNA Mismatch Repair
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| Creator |
Bhakat, Kishore K
Sharma N, Sudarshan Das, Jyotirmoy |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A new gene, mutK, of Vibrio cholerae, encoding a 19-kDa protein which is involved in repairing mismatches
in DNA via a presumably methyl-independent pathway, has been identified. The product of the mutK gene
cloned in either high- or low-copy-number vectors can reduce the spontaneous mutation frequency of Escherichia
coli mutS, mutL, mutU, and dam mutants. The spontaneous mutation frequency of a chromosomal mutK
knockout mutant was almost identical to that of wild-type V. cholerae cells, indicating that when the methyldirected
mismatch repair is blocked, the repair potential of MutK becomes apparent. The complete nucleotide
sequence of the mutK gene has been determined, and the deduced amino acid sequence showed three open
reading frames (ORFs), of which the ORF3 represents the mutK gene product. The mutK gene product has no
significant homology with any of the proteins deposited in the EMBL data bank. ORF2, located upstream of
mutK, encodes a 14-kDa protein which has more than 70% homology with a hypothetical protein found only
downstream of the E. coli vsr gene. ORF1, located farther upstream of mutK, has more than 80% homology with
a major cold shock protein found in several bacteria. Downstream of mutK, a partial ORF having 60%
homology with an RNA methyltransferase has been identified. The mutK gene has recently been positioned in
the ordered cloned DNA map of the genome of the V. cholerae strain from which the gene was isolated (10).
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| Publisher |
American Society for Microbiology
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/680/1/JOURNAL_OF_BACTERIOLOGY__181(_3)_879%2D883;1999[78].pdf
Bhakat, Kishore K and Sharma N, Sudarshan and Das, Jyotirmoy (1999) The mutK Gene of Vibrio cholerae: a New Gene Involved in DNA Mismatch Repair. Journal of Bacteriology, 181 (3). pp. 879-883. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/680/ |
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