Thyroid Hormone-Induced Maturation of Astrocytes Is Associated with the Expression of New Variants of Vimentin and Their Phosphorylation
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Thyroid Hormone-Induced Maturation of Astrocytes Is
Associated with the Expression of New Variants of Vimentin
and Their Phosphorylation
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| Creator |
Paul, Surojit
Gharami, Kusumika Das, Sumantra Sarkar, Pranab Kumar |
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| Subject |
Cell Biology & Physiology
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| Description |
The role of thyroid hormone (TH)-induced vimentin
expression in promoting the maturation of astrocytes
has been investigated. Comparative immunocytochemical
staining with vimentin antibody demonstrated
distinctly different patterns of expression of vimentin during
the progressive maturation of astrocytes of normal
and TH-deficient astrocyte cultures. [35S]Methionine labeling
of cells showed a significant decline in the level of
vimentin in the hypothyroid cultures at all ages from day
5 to 20. Western blot analysis suggested that the maturation
of normal astrocytes was associated with the appearance
of several acidic and basic variants of vimentin,
whose expression was significantly delayed or reduced in
the TH-deficient astrocytes. In normal astrocyte cultures,
two acidic variants of vimentin, one of similar molecular
weight and the other of lower molecular weight, were
found to be phosphorylated during the final transformation
of the epithelioid form into the mature stellate form.
None of these phosphorylated forms was evident in the
TH-deficient astrocytes. This was further confirmed by
negative immunocytochemical staining of 5- to 18-dayold
cultures of TH-deficient astrocytes with antibody
TM71, specific to phosphorylated vimentin, compared
with age-matched normal astrocytes, which displayed
consistent positive staining. The overall results indicate
that TH-induced expression of phosphorylated forms of
vimentin may be essential for the intracellular organization
of the cytoskeleton in a way conducive to the morphological
maturation of astrocytes. Key Words: Astrocytes—
Morphology—Hypothyroidism—Vimentin—
Variants—Phosphorylation.
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| Publisher |
Blackwell Publishing
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/690/1/JOURNAL_OF_NEUROCHEMISTRY__73_(5)1964%2D1972;1999[9].pdf
Paul, Surojit and Gharami, Kusumika and Das, Sumantra and Sarkar, Pranab Kumar (1999) Thyroid Hormone-Induced Maturation of Astrocytes Is Associated with the Expression of New Variants of Vimentin and Their Phosphorylation. Journal Of Neurochemistry, 73 (5). pp. 1964-1972. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/690/ |
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