Maturation-dependent modification of the protein phosphorylation profile of isolated goat sperm plasma membrane
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Maturation-dependent modification of the protein phosphorylation
profile of isolated goat sperm plasma membrane
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| Creator |
Nath, Debjani
Majumder, Gopal Chandra |
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| Subject |
Cell Biology & Physiology
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| Description |
Highly purified plasma membranes, isolated by an aqueous two-phase polymer
method from goat epididymal spermatozoa, were found to possess a kinase activity
that causes phosphorylation of serine and threonine residues of several endogenous
plasma membrane proteins. Cyclic AMP, cyclic GMP, Ca2+\p=n-\calmodulin,
phosphatidylserine\p=n-\diolein,polyamines and heparin had no appreciable effect on this
kinase. Autoradiographic analysis showed that the profile of the phosphorylation of
membrane proteins by this endogenous cAMP-independent protein kinase underwent
marked modulation during the transit of spermatozoa through the epididymis. In
caput sperm plasma membrane, 18, 21, 43, 52, 74 and 90 kDa proteins were
phosphorylated, whereas, in the corpus and cauda epididymal spermatozoa, a
differential phosphorylation pattern was observed with respect to the 90, 74, 21 and 18
kDa proteins. The rate of phosphorylation of the 74 kDa protein decreased markedly
during the early phase of sperm maturation (caput to distal corpus epididymides)
whereas there was little change in kinase activity in sperm plasma membrane. In
contrast, the rates of phosphorylation of the 18 and 21 kDa proteins increased during
the terminal phase (distal corpus to distal cauda epididymides) of sperm maturity,
although the kinase activity of membrane decreased significantly during this phase.
The modulation of the phosphorylated states of these specific membrane proteins may
play an important role in the maturation of epididymal spermatozoa
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/701/1/JOURNAL_OF_REPRODUCTION_AND_FERTILITY_115_(_1)_29%2D37;1999[89].pdf
Nath, Debjani and Majumder, Gopal Chandra (1999) Maturation-dependent modification of the protein phosphorylation profile of isolated goat sperm plasma membrane. Journal of Reproduction and Fertility, 115 (1). pp. 29-37. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/701/ |
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