Identification of 9-O acetyl sialoglycoconjugates (9-OAcSGs) as biomarkers in childhood acute lymphoblastic leukemia using a lectin, AchatininH, as a probe
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Identification of 9-O acetyl sialoglycoconjugates (9-OAcSGs) as biomarkers in
childhood acute lymphoblastic leukemia using a lectin, AchatininH, as a probe
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| Creator |
Sinha, D
Mandal, Chitra Bhattacharyya, DK |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Neoplastic transformation causes changes in cell surface
architecture, most notably, aberrant sialylation. Exploiting the
restricted specificity of a 9-O acetyl sialic acid (9-OAcSA) binding
lectin, AchatininH (ATNH), we have identified two 9-O acetyl
sialoglyconjugates (9-OAcSGs) on lymphoblasts of 87 children
suffering from acute lymphoblastic leukemia (ALL). The preferential
binding of ATNH to lymphoblasts induces their 11-fold
increased agglutination (81 ± 7.8%) compared to peripheral
blood mononuclear cells (PBMC) of normal donors (8 ± 4.3%)
which corroborates with flow cytometry studies. Agglutination
of MOLT-4 (87 ± 4.8%), a lymphoblastoid cell line and MDCK
(91.25 ± 0.01%), a cell line expressing surface 9-OAcSA, confirms
the preferential binding of ATNH to lymphoblasts through
their surface 9-OAcSGs. Furthermore, fluorometric quantitation
reveals a 4.6-fold increase in % of 9-OAcSA on lymphoblasts
of ALL patients (42.1 ± 4.1%) compared to normal donors
(9.2. ± 3.4%). Western blotting confirms that ATNH recognizes
two membrane sialoglycoconjugates, of MW 120 kDa and
90 kDa, both having 9-OAcSA a2 ! 6 GalNAc terminal sugar
moiety as their lectinogenic epitope. We propose that these 9-
OAcSGs may serve as biomarkers for detection and monitoring
of lymphoblasts in ALL and accordingly merit therapeutic considerations.
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| Publisher |
Nature Publishing Group
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/708/1/LEUKEMIA_13_(1)119%2D125;1999[88].pdf
Sinha, D and Mandal, Chitra and Bhattacharyya, DK (1999) Identification of 9-O acetyl sialoglycoconjugates (9-OAcSGs) as biomarkers in childhood acute lymphoblastic leukemia using a lectin, AchatininH, as a probe. Leukemia, 13 (1). pp. 119-125. |
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| Relation |
http://dx.doi.org/10.1038/sj.leu.2401239
http://www.eprints.iicb.res.in/708/ |
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