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Import of RNA into Leishmania Mitochondria Occurs through Direct Interaction with Membrane-bound Receptors

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Import of RNA into Leishmania Mitochondria Occurs through Direct Interaction with Membrane-bound Receptors
 
Creator Mahapatra, Sridam
Adhya, Samit
 
Subject Molecular & Human Genetics
 
Description Cytoplasmic tRNAs are imported into the kinetoplast mitochondrion of Leishmania, but the mechanism of import is unknown, particularly whether RNA is transferred as a ribonucleoprotein complex through the protein import pathway or by a distinct receptor-mediated mechanism. Using isolated mitochondria, it was shown that a small, importable RNA, which is structurally homologous to tRNA, binds rapidly, specifically, and with high affinity to the mitochondrial surface in the absence of soluble protein factors to form an import intermediate. Two classes of binding site of apparent Kd 0.3 and 10 nM, respectively, were distinguished. tRNA from Leishmania, but not yeast, competitively inhibited the binding. Northwestern blot analysis revealed the presence of a 15-kDa RNA binding protein on the mitochondrial surface. Whereas receptor binding was resistant to heparin and KCl, internalization was sensitive to both reagents. These results are consistent with the presence of a direct mechanism of receptor-mediated RNA import on Leishmania mitochondria.
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/715/1/JOURNAL_OF_BIOLOGICAL_CHEMISTRY%2C_271(_34)%2C_20432%2D20437[24].pdf
Mahapatra, Sridam and Adhya, Samit (1996) Import of RNA into Leishmania Mitochondria Occurs through Direct Interaction with Membrane-bound Receptors. The Journal of Biological Science, 271 (34). pp. 20432-20437.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/715/