The Role of Amoebocytes in Endotoxin-Mediated Coagulation in the Innate Immunity of Achatina fulica Snails
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
The Role of Amoebocytes in Endotoxin-Mediated Coagulation in the
Innate Immunity of Achatina fulica Snails
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| Creator |
Biswas, C
Mandal, Chitra |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Biswas C, Mandal C. The Role of Amoebocytes in Endotoxin-Mediated Coagulation in the Innate Immunity
of Achatina fulica Snails. Scand J Immunol 1999;49:131–138
Achatina amoebocyte lysate (AAL) derived from amoebocytes of Achatina fulica was activated by Gramnegative
bacterial endotoxins in a time-dependent manner resulting in gel formation/coagulation. The
activation and maximum proliferation of amoebocytes was observed 40 min after intramuscular injection
(20 mg/snail) of endotoxin. Endotoxin-mediated proteolytic activity of AAL towards a serine-protease-specific
chromogenic substrate was maximum at pH 8.0, 378C and within 15 min in a divalent-cation-dependent
manner. The AAL activity induced by the endotoxin was directly dependent on the endotoxin concentration,
showed a high specificity and saturated at higher endotoxin concentrations. An endotoxin-sensitive factor
(ESF) was purified from AAL to apparent homogeneity by single-step affinity chromatography on a heparin-
Sepharose 4B column. Native ESF of molecular weight 140 000 was composed of two identical subunits of
molecular weight 70 000 attached through non-covalent association. A strong binding to endotoxin
(Escherichia coli 055:B5) was exhibited by ESF with a 40-fold higher biological activity than AAL. The
ESF was shown to have a unique Phe–Ile active site with regard to its alternate activation by a-chymotrypsin
instead of endotoxin. The ESF was characterized as a serine protease type as evidenced by potent inhibition
with specific inhibitors.
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| Publisher |
Blackwell Publishing
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/727/1/SCANDINAVIAN_JOURNAL_OF_IMMUNOLOGY__49(_2)_131%2D138;1999[76].pdf
Biswas, C and Mandal, Chitra (1999) The Role of Amoebocytes in Endotoxin-Mediated Coagulation in the Innate Immunity of Achatina fulica Snails. Scandinavian Journal Of Immunology, 49 (2). pp. 131-138. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/727/ |
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