Chemokine-Induced Leishmanicidal Activity in Murine Macrophages via the Generation of Nitric Oxide
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Chemokine-Induced Leishmanicidal Activity in Murine Macrophages
via the Generation of Nitric Oxide
|
|
| Creator |
Bhattacharyya, Sandip
Ghosh, Sanjukta Dasgupta, Biplab Mazumder, Debashis Roy, Syamal Majumdar, Subrata |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
This study explored the role of the proinflammatory chemokines macrophage inflammatory
protein (MIP)–1a and macrophage chemoattractant protein (MCP)–1 for development of antileishmanial
activity. There was substantial inhibition in nitrite generation in Leishmania donovani–
infected macrophages. A marked elevation of nitrite generation and induction of inducible
nitric oxide (NO) synthase (iNOS) mRNA was found in chemokine-primed parasite-infected
macrophages. Tumor necrosis factor–a, which is the priming signal for NO production, was
also up-regulated under similar experimental conditions. The priming with chemokine inhibited
the multiplication of L. donovani amastigotes within the intramacrophageal milieu. The antileishmanial
effect of chemokines was almost completely abrogated when the macrophages were
preincubated with L-N-monomethyl arginine, the specific inhibitor of iNOS. The results of this
investigation suggest that the CC chemokinesMIP-1a andMCP-1 orchestrate an antileishmanial
armamentarium via the induction of an NO-mediated regulatory mechanism to control the intracellular
growth and multiplication of the Leishmania protozoan.
|
|
| Publisher |
University of Chicago Press
|
|
| Date |
2002
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/741/1/JOURNAL_OF_INFECTIOUS_DISEASES_185(12)1704%2D1708;2002[47].pdf
Bhattacharyya, Sandip and Ghosh, Sanjukta and Dasgupta, Biplab and Mazumder, Debashis and Roy, Syamal and Majumdar, Subrata (2002) Chemokine-Induced Leishmanicidal Activity in Murine Macrophages via the Generation of Nitric Oxide. The Journal of Infectious Diseases, 185 (12). pp. 1704-1708. |
|
| Relation |
http://dx.doi.org/10.1086/340820
http://www.eprints.iicb.res.in/741/ |
|