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Regulation of Cellobiase Secretion in Termitomyces clypeatus by Co-Aggregation with Sucrase

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Regulation of Cellobiase Secretion in Termitomyces clypeatus by Co-Aggregation with Sucrase
 
Creator Mukherjee, Sumana
Khowala, Suman
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Regulated secretory proteins are sorted via selective co-aggregation in eukaryotes. Cellobiase (C) of the filamentous fungus Termitomyces clypeatus remained co-aggregated with sucrase (S), and only one isoform of each of the enzymes was present in intra- and extracellular extracts. Kinetics of secretion of sucrase increased in vivo and in vitro in secreting (Sc) medium and decreased under non-secreting (NSc) conditions similar to those observed for cellobiase. In the Sc condition, total enzyme production and activity ratios of cellobiase and sucrase (C/S) in cell-bound, extra- and intracellular preparations increased with time and were significantly higher from those obtained in non-secretory media. It was concluded that secretion of sucrase in culture medium is under same cellular regulation as that of cellobiase, and sucrase is involved in regulating extracellular release of cellobiase through co-aggregation in the fungus.
 
Publisher Springer Verlag
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/752/1/CURRENT_MICROBIOLOGY_45_(1)_70%2D73;2002[44].pdf
Mukherjee, Sumana and Khowala, Suman (2002) Regulation of Cellobiase Secretion in Termitomyces clypeatus by Co-Aggregation with Sucrase. Current Microbiology, 45 (1). pp. 70-73.
 
Relation http://dx.doi.org/10.1007/s00284-001-0079-4
http://www.eprints.iicb.res.in/752/