Regulation of Cellobiase Secretion in Termitomyces clypeatus by Co-Aggregation with Sucrase
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Regulation of Cellobiase Secretion in Termitomyces clypeatus
by Co-Aggregation with Sucrase
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Creator |
Mukherjee, Sumana
Khowala, Suman |
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Subject |
Drug Development/Diagnostics & Biotechnology
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Description |
Regulated secretory proteins are sorted via selective co-aggregation in eukaryotes. Cellobiase
(C) of the filamentous fungus Termitomyces clypeatus remained co-aggregated with sucrase (S), and only
one isoform of each of the enzymes was present in intra- and extracellular extracts. Kinetics of secretion
of sucrase increased in vivo and in vitro in secreting (Sc) medium and decreased under non-secreting
(NSc) conditions similar to those observed for cellobiase. In the Sc condition, total enzyme production
and activity ratios of cellobiase and sucrase (C/S) in cell-bound, extra- and intracellular preparations
increased with time and were significantly higher from those obtained in non-secretory media. It was
concluded that secretion of sucrase in culture medium is under same cellular regulation as that of
cellobiase, and sucrase is involved in regulating extracellular release of cellobiase through co-aggregation
in the fungus.
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Publisher |
Springer Verlag
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Date |
2002
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/752/1/CURRENT_MICROBIOLOGY_45_(1)_70%2D73;2002[44].pdf
Mukherjee, Sumana and Khowala, Suman (2002) Regulation of Cellobiase Secretion in Termitomyces clypeatus by Co-Aggregation with Sucrase. Current Microbiology, 45 (1). pp. 70-73. |
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Relation |
http://dx.doi.org/10.1007/s00284-001-0079-4
http://www.eprints.iicb.res.in/752/ |
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