High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
High affinity binding between laminin and laminin binding protein
of Leishmania is stimulated by zinc and may involve laminin
zinc-finger like sequences
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| Creator |
Bandyopadhyay, Keya
Karmakar, Sudipan Ghosh, Abhijit Das, Pijush K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
In the course of trying to understand the pathogenesis of
leishmaniasis in relation to extracellular matrix (ECM)
elements, laminin, a major ECM protein, has been found to
bind saturably and with high a�nity to a 67-kDa cell surface
protein of Leishmania donovani. This interaction involves a
single class of binding sites, which are ionic in nature,
conformation-dependent and possibly involves sulfhydryls.
Binding activity was signi®cantly enhanced by Zn2+, an
effect possibly mediated through Cys-rich zinc ®nger-like
sequences on laminin. Inhibition studies with monoclonals
against polypeptide chains and speci®c peptides with adhe-
sive properties revealed that the binding site was localized in
one of the nested zinc ®nger consensus sequences of B1 chain containing the speci®c pentapeptide sequence, YIGSR.
Furthermore, incubation of L. donovani promastigotes with
C(YIGSR)3-NH2 peptide amide or antibody directed
against the 67-kDa laminin-binding protein (LBP) induced
tyrosine phosphorylation of proteins with a molecular mass
ranging from 115 to 130 kDa. These studies suggest a role
for LBP in the interaction of parasites with ECM elements,
which may mediate one or more downstream signalling
events necessary for establishment of infection.
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| Publisher |
Blackwell Publishing
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| Date |
2002
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/753/1/EUROPEAN_JOURNAL_OF_BIOCHEMISTRY_269(_17)_4351%2D4358;2002[29].pdf
Bandyopadhyay, Keya and Karmakar, Sudipan and Ghosh, Abhijit and Das, Pijush K (2002) High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences. European Journal Of Biochemistry, 269 (6). pp. 1622-1629. |
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| Relation |
http://dx.doi.org/10.1046/j.1432-1327.2002.02793.x
http://www.eprints.iicb.res.in/753/ |
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