Human placental extract: A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities
IR@IICB: CSIR-Indian Institute of Chemical Biology, KolkataView Archive Info
Human placental extract:
A human placental extract: in vivo and in vitro assessments of its
melanocyte growth and pigment-inducing activities
Dutta, Ajit Kr
Dutta, Pijush K
Infectious Diseases and Immunology
The authenticity of various prototype human placental extracts with biological
activity, such as that inducing vitiligo repigmentation, is under serious criticism, mainly due to a
lack of demonstration at the cellular level. Considering the present worldwide scenario with
regard to the occurrence and treatment of vitiligo, a thorough scientific exploration of such
extracts should be undertaken.
One such prototype placental preparation was prepared, and was evaluated with
regard to its melanogenic action in C57BL/6J mice in vivo and its mitogenic and melanogenic
activity on B16F10 mouse melanoma cells and normal human melanocytes in vitro. The extract
was applied topically to mice with age-induced prolonged telogenic phase of hair growth (grey
body coat hair). Standard 3H-thymidine incorporation and spectrophotometric methods were
followed to illustrate mitogenic and melanogenic effects at the cellular level.
The resurgence of blue skin, followed by shiny black hair, at the regions of application
of the extract demonstrated the reversal of the age-induced prolonged telogenic phase of hair
growth to the anagenic phase after topical application of the extract on C57BL/6J mice. Further
support was obtained from histology where, at the extract-treated sites, the development of new
melanogenic centers and hair follicles was observed. During in vitro studies, the vehicle-free
extract constituents stimulated both mitogenesis and melanogenesis of B16F10 mouse
melanoma cells in a concentration-dependent manner. The cell morphology and extent of
melanogenesis also showed significant changes. In addition, two known melanocyte activitymodulating
peptides, endothelin-1 (ET-1) and adrenocorticotropic hormone (ACTH), were
determined in the extract, chiefly in the total lipid fraction, indicating their effective cutaneous
The extract was found to be a potent mitogen in the in vitro condition and a potent
melanogen in both the in vitro and in vivo situations. This strongly suggests its therapeutic
potential for the repigmentation of vitiligo patches.
Pal, Prajnamoy and Mallick, Shampa and Mandal, Samir and Das, Madhusudan and Dutta, Ajit Kr and Dutta, Pijush K and Bhadra, Ranjan (2002) Human placental extract: A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities. International Journal Of Dermatology, 41 (11). pp. 760-767.