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Human placental extract: A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Human placental extract: A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities
 
Creator Pal, Prajnamoy
Mallick, Shampa
Mandal, Samir
Das, Madhusudan
Dutta, Ajit Kr
Dutta, Pijush K
Bhadra, Ranjan
 
Subject Infectious Diseases and Immunology
 
Description The authenticity of various prototype human placental extracts with biological activity, such as that inducing vitiligo repigmentation, is under serious criticism, mainly due to a lack of demonstration at the cellular level. Considering the present worldwide scenario with regard to the occurrence and treatment of vitiligo, a thorough scientific exploration of such extracts should be undertaken. One such prototype placental preparation was prepared, and was evaluated with regard to its melanogenic action in C57BL/6J mice in vivo and its mitogenic and melanogenic activity on B16F10 mouse melanoma cells and normal human melanocytes in vitro. The extract was applied topically to mice with age-induced prolonged telogenic phase of hair growth (grey body coat hair). Standard 3H-thymidine incorporation and spectrophotometric methods were followed to illustrate mitogenic and melanogenic effects at the cellular level. The resurgence of blue skin, followed by shiny black hair, at the regions of application of the extract demonstrated the reversal of the age-induced prolonged telogenic phase of hair growth to the anagenic phase after topical application of the extract on C57BL/6J mice. Further support was obtained from histology where, at the extract-treated sites, the development of new melanogenic centers and hair follicles was observed. During in vitro studies, the vehicle-free extract constituents stimulated both mitogenesis and melanogenesis of B16F10 mouse melanoma cells in a concentration-dependent manner. The cell morphology and extent of melanogenesis also showed significant changes. In addition, two known melanocyte activitymodulating peptides, endothelin-1 (ET-1) and adrenocorticotropic hormone (ACTH), were determined in the extract, chiefly in the total lipid fraction, indicating their effective cutaneous permeation. The extract was found to be a potent mitogen in the in vitro condition and a potent melanogen in both the in vitro and in vivo situations. This strongly suggests its therapeutic potential for the repigmentation of vitiligo patches.
 
Publisher Blackwell Publishing
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/759/1/INTERNATIONAL_JOURNAL_OF_DERMATOLOGY__41_(11)_760%2D767;2002.pdf
Pal, Prajnamoy and Mallick, Shampa and Mandal, Samir and Das, Madhusudan and Dutta, Ajit Kr and Dutta, Pijush K and Bhadra, Ranjan (2002) Human placental extract: A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities. International Journal Of Dermatology, 41 (11). pp. 760-767.
 
Relation http://dx.doi.org/10.1046/j.1365-4362.2002.01524.x
http://www.eprints.iicb.res.in/759/