The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium
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| Creator |
Mohanakumar, K P
Samanta Ray, S Biisselberg, D |
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| Subject |
Cell Biology & Physiology
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| Description |
Effects of the neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its metabolite, 1-methyl-4-phenyl pyridinium (MPP+) were investigated on neuronal membrane currents and intrasynaptosomal calcium levels employing whole cell patch clamp and FURA-2AM respectively. Membrane currents elicited by voltage ramps as well as voltage activated potassium-currents were studied in the cultured rat dorsal root ganglia (DRG) neurons. These neurons contained 0.36 ± 0.04 pmol/mg of dopamine as detected employing HPLC-electrochemistry. MPTP and MPP+ (1 μM) respectively caused significant increase (25 ± 4%) or inhibition (53 ± 10%) of the total membrane current. Potassium-currents elicited by voltage jumps from –80 to 0 mV were significantly inhibited (68 ± 8%) by MPP+, but MPTP caused no effect. MPP+-induced increase in the FURA-2 fluorescence signal was insensitive to extrasynaptosomal calcium, indicated intrasynaptosomal mobilization by the neurotoxin. These results suggest involvement of ion channels in MPTP neurotoxicity, and imply its role in the etiopathology of Parkinson’s disease
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| Publisher |
John Wiley & Sons
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| Date |
2002
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/777/1/NEUROSCIENCE_RESEARCH_COMMUNICATIONS__30(_1_)_35%2D42;2002[82].pdf
Mohanakumar, K P and Samanta Ray, S and Biisselberg, D (2002) The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium. Neuroscience Research Communications, 30 (1). pp. 35-42. ISSN 0893-6609 |
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| Relation |
http://dx.doi.org/10.1002/nrc.10015
http://www.eprints.iicb.res.in/777/ |
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