Molecular characterization of a distinct bipartite begomovirus species infecting tomato in India
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Molecular characterization of a distinct bipartite begomovirus species infecting tomato in India
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| Creator |
Hallan, Vipin
Kumar, Y Zaidi, A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
A distinct bipartite begomovirus was found
associated with tomato plants showing yellowing, curling,
and crumpling of the leaves, in a sub-temperate region in
India. The complete DNA-A and DNA-B components were
amplified through rolling circle amplification (RCA) using
U-29 DNA polymerase and characterized. The DNA-A of
the isolate was comprised of 2,756 nucleotides, encoding
six open reading frames (ORFs) and DNA-B that of 2,725
nucleotides, encoding two ORFs. Genome organization of
the isolate was typical of an old world bipartite begomovirus. Comparisons showed that DNA-A and its intergenic region (IR) have the highest sequence identity (86% and 84%, respectively) with the Tomato leaf curl New Delhi virus (ToLCNDV; DQ116885) and some other begomoviruses
([84%) reported from cucurbits and tomato. This
data suggested that the isolate is a distinct begomovirus
species for which a name Tomato leaf curl Palampur virus
(ToLCPMV) is proposed. DNA-B showed the maximum
sequence identity (73%) with Tomato leaf curl New Delhi
virus-India-[Pakistan:Dargai:T5/6:2001] (AY150305). The
common region (CR) of DNA-A and DNA-B showed 94%
sequence similarity with each other. In the present study,
phylogenetic relationship of this new species was also
established with different begomoviruses reported from
tomato and other begomoviruses showing highest homologies
with complete DNA-A and DNA-B sequences. ToLCPMV is being reported from a sub-temperate region in India which was previously unaffected by begomoviruses and its whitefly vector.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/12/1/2008_Molecular.pdf
Hallan, Vipin and Kumar, Y and Zaidi, A A (2008) Molecular characterization of a distinct bipartite begomovirus species infecting tomato in India. Virus Genes, 37 (3). pp. 425-431. |
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| Relation |
http://ihbt.csircentral.net/12/
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