Isolation of a Psychrotrophic Exiguobacterium sp. SKPB5 (MTCC 7803) and Characterization of Its Alkaline Protease
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Isolation of a Psychrotrophic Exiguobacterium sp. SKPB5 (MTCC 7803)
and Characterization of Its Alkaline Protease
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| Creator |
Kasana , Ramesh C
Yadav , S K |
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| Subject |
Microbiology
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| Description |
Out of nine psychrotrophic bacterial strains isolated from cold environments of the Western
Himalayas, SKPB5 was selected for protease purification and characterization because it had the largest
zone of clearance on plate assay. On the basis of the phenotypic and biochemical characterization and
16S rRNA gene-sequencing studies, isolate was identified as Exiguobacterium sp. SKPB5. The protease
was purified near to homogeneity with a purification fold of 7.1, and its molecular weight was determined
to be 36 kDa. The enzyme exhibited maximum stability at 50 degree C and an optimal pH of 8.0. Metal
ions Mg2+, Ca2+, Zn2+, and Mn2+ enhanced the enzyme activity, whereas Cu2+ had no effect. Phenylmethanesulfonyl
fluoride and ethylenediaminetetraacetic acid did not show any effect on the activity
of the enzyme, whereas a 20% increase in activity was observed when it was incubated in presence of
reducing agents such as b-mercaptoethanol and dithiothreitol. This suggests that the protease isolated
from psychrotrophic Exiguobacterium sp. SKPB5 belongs to the cysteine family. The results highlight
the relevance of unexplored microbes from cold environments of Western Himalayas for the isolation of
protease enzymes active at wide range of temperature and pH.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/18/1/2007_Isolation_224%2D229_vol.54.pdf
Kasana , Ramesh C and Yadav , S K (2007) Isolation of a Psychrotrophic Exiguobacterium sp. SKPB5 (MTCC 7803) and Characterization of Its Alkaline Protease. Current Microbiology, 54 (3). pp. 224-229. |
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| Relation |
http://ihbt.csircentral.net/18/
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