Cydonia oblonga as reservoir of Apple chlorotic leaf spot virus in India
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Cydonia oblonga as reservoir of Apple chlorotic leaf spot virus in India
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| Creator |
Rana, T
Hallan, Vipin Chandel, V Zaidi, A A |
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| Subject |
Plant viruses
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| Description |
Quince (Cydonia oblonga ) has traditionally been used as medicine and flavouring agent (Kartikar et al.
, 1981). Also it is a valued dwarfing rootstock for pear which produces more fruit-bearing branches and has accelerated fruit maturity when used in this way. Quince is a natural host to several virus and virus-like diseases infecting apple and other rosaceous species, though it has not been described as primary host for any virus (Sutic
et al., 1999). Apple chlorotic leaf spot virus
(ACLSV), type species of the genus Trichovirus (Martelli
et al. , 1994), induces severe graft incompatibilities in some Prunus spp. combinations, causing major problems in nurseries. In surveys conducted in Salooni valley, Himachal Pradesh, India, leaves showing distortion and yellow spots were collected from quince grown between the apple orchards. Preliminary testing was done using ELISA for
ACLSV (Agdia), Apple mosaic virus and Apple stem grooving virus (Loewe) as per the manufacturers’ instruction. Only ACLSV antibodies reacted positively. To rule out possibility of other viruses, host-range studies
were carried out. Chenopodium amaranticolor
showed small chlorotic spots in inoculated leaves. A single lesion was inoculated onto C. quinoa which gave chlorotic and necrotic spots on inoculated leaves, followed by
chlorotic spots in upper leaves. For further confirmation degenerate primers for amplification of complete coat protein and part of 3 ′ UTR region of ACLSV were designed (Accession Nos. AM490253 and AM490254). RT-PCR using these primers gave an amplicon of approximately 800 bp which was cloned and sequenced (Acc. No. AM498049).
The nucleotide sequence was analysed and compared with the partial coat protein of ACLSV reported in quince from Greece (Acc. No. AM292923). Pairwise comparison was performed using B12seq program. The sequence exhibited nucleotide and amnio acid similarity levels of 84 and 87% respectively which is in accordance to the accepted range of
variability within species (Adams et al
., 2004). This is the first report
confirming the presence of ACLSV in quince from India and first complete
coat protein of ACLSV from this host.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/317/2/381_2008_Cydonia.pdf
Rana, T and Hallan, Vipin and Chandel, V and Zaidi, A A (2008) Cydonia oblonga as reservoir of Apple chlorotic leaf spot virus in India. Plant Pathology, 57. p. 393. |
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| Relation |
http://ihbt.csircentral.net/317/
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