CSIR Central

Use of an Attenuated Leishmanial Parasite as an Immunoprophylactic and Immunotherapeutic Agent against Murine Visceral Leishmaniasis

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

View Archive Info
 
 
Field Value
 
Title Use of an Attenuated Leishmanial Parasite as an Immunoprophylactic and Immunotherapeutic Agent against Murine Visceral Leishmaniasis
 
Creator Mukhopadhyay, Srirupa
Bhattacharyya, Sandip
Majhi, Ramdhan
De, Tripti
Naskar, Khudiram
Majumdar, Subrata
Roy, Syamal
 
Subject Infectious Diseases and Immunology
 
Description The ability of the leishmanial parasite UR6 to act as an immunoprophylactic and immunotherapeutic agent against Leishmania donovani infection in BALB/c mice was investigated. Unlike the virulent L. donovani AG83 (MOHOM/IN/1983/AG83), UR6 given through intracardiac route failed to induce visceral infection, but when it was injected subcutaneously, UR6 induced a short-lived and localized self-healing skin lesion. Priming of peritoneal macrophages with UR6 in vitro induced superoxide (O2 2) generation, whereas similar experiments with virulent AG83 inhibited O2 2 generation. It was observed that priming of mice with either live or sonicated UR6 in the absence of any adjuvant provided strong protection against subsequent virulent challenge. Further, UR6-primed infected mice not only displayed a strong antileishmanial delayed-type hypersensitivity (DTH) response but also showed an elevated level of the serum antileishmanial immunoglobulin G2a (IgG2a) isotype, whereas infected mice failed to mount any antileishmanial DTH response and showed an elevated level of IgG1. This indicates that UR6 priming and subsequent L. donovani infection allowed the expansion of Th1 cells. Our studies indicate that UR6 has potential to be used as an immunoprophylactic and immunotherapeutic agent against experimental visceral leishmaniasis.
 
Publisher American Society for Microbiology
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/809/1/CLINICAL_AND_DIAGNOSTIC_LABORATORY_IMMUNOLOGY_7_(2)_233%2D240;2000[53].pdf
Mukhopadhyay, Srirupa and Bhattacharyya, Sandip and Majhi, Ramdhan and De, Tripti and Naskar, Khudiram and Majumdar, Subrata and Roy, Syamal (2000) Use of an Attenuated Leishmanial Parasite as an Immunoprophylactic and Immunotherapeutic Agent against Murine Visceral Leishmaniasis. Clinical and Diagnostic Laboratory Immunology, 7 (2). pp. 233-240.
 
Relation http://dx.doi.org/10.1128/CDLI.7.2.233-240.2000
http://www.eprints.iicb.res.in/809/