Role of an RNA-binding Protein in Import of tRNA into Leishmania Mitochondria
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Role of an RNA-binding Protein in Import of tRNA into
Leishmania Mitochondria
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| Creator |
Adhya, Samit
Ghosh, Trina Das, Anish Bera, Saphal Kanti Mahapatra, Sridam |
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| Subject |
Molecular & Human Genetics
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| Description |
Nuclear-encoded cytoplasmic tRNAs are imported
into the mitochondria of kinetoplastid protozoa by an
unknown mechanism. In a Leishmania in organello system,
ATP-dependent import of a cloned, unspliced
tRNATyr(GUA) transcript was demonstrated by protection
from ribonuclease, whereas import of a tRNAGln
-
(CUG) transcript was much less efficient. Specific binding
of tRNATyr to two mitochondrial surface proteins of
15 and 22 kilodaltons was observed. Tubulin antisensebinding
protein (TAB), the 15-kilodaton species, was purified
to apparent homogeneity by RNA affinity chromatography.
TAB forms stable complexes with the D stemloop
region of tRNATyr. Immunocytochemical and cell
fractionation experiments, combined with limited proteolysis,
suggested the association of TAB with the outer
mitochondrial membrane. Importantly, anti-TAB antibody
specifically inhibited binding as well as import of
tRNATyr and of a synthetic structural homolog. These
results support the role of TAB as a membrane-bound
receptor or carrier for RNA import into Leishmania
mitochondria.
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| Date |
1997
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/815/1/JOURNAL_OF_BIOLOGICAL_CHEMISTRY%2C_272(_34)%2C_21396%2D21402[35].pdf
Adhya, Samit and Ghosh, Trina and Das, Anish and Bera, Saphal Kanti and Mahapatra, Sridam (1997) Role of an RNA-binding Protein in Import of tRNA into Leishmania Mitochondria. The Journal of Biological Chemistry, 272 (34). pp. 21396-21402. |
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| Relation |
http://dx.doi.org/10.1074/jbc.272.34.21396
http://www.eprints.iicb.res.in/815/ |
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