Comparison of Global Transcription Responses allows Identification of Vibrio Cholerae Genes Differentially Expressed following Infection
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Comparison of Global Transcription Responses allows Identification of
Vibrio Cholerae Genes Differentially Expressed following Infection
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| Creator |
Das, Soumita
Chakrabortty, Amit Banerjee, Rajat Roychoudhury, Susanta Chaudhuri, Keya |
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| Subject |
Molecular & Human Genetics
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| Description |
Comparison of global transcription profiles of Vibrio cholerae grown in vitro and in vivo revealed that 20% of the genome was repressed
and about 5% was induced under in vivo conditions. Hybridization with the cloned genes revealed that the virulence genes ctx, toxR, toxT
and tcpA were induced under in vivo conditions. Dissection of two in vivo induced cosmids identified another set of three genes homologous
to cheY1 involved in motility and chemotaxis, pnuC encoding the major component of the nicotinamide mononucleotide transport system
and icmF belonging to a cassette involved in multiplication inside host cells. These results demonstrate that the global transcription profile
approach might be a powerful method for identification of differentially expressed transcripts under in vivo conditions. ß 2000 Federation
of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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| Publisher |
Elsevier
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/820/1/FEMS_MICROBIOLOGY_LETTERS__190(_1)87%2D91;2000[21].pdf
Das, Soumita and Chakrabortty, Amit and Banerjee, Rajat and Roychoudhury, Susanta and Chaudhuri, Keya (2000) Comparison of Global Transcription Responses allows Identification of Vibrio Cholerae Genes Differentially Expressed following Infection. FEMS Microbiology Letters, 190 (1). pp. 87-91. |
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| Relation |
http://dx.doi.org/10.1111/j.1574-6968.2000.tb09267.x
http://www.eprints.iicb.res.in/820/ |
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