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Role of Linkage Specific 9-O-Acetylated Sialoglycoconjugates in Activation of the Alternate Complement Pathway in Mammalian Erythrocytes

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Role of Linkage Specific 9-O-Acetylated Sialoglycoconjugates in Activation of the Alternate Complement Pathway in Mammalian Erythrocytes
 
Creator Sharma, Vineeta
Chatterjee, Mitali
Sen, Goutam
Chava, Anil Kumar
Mandal, Chitra
 
Subject Infectious Diseases and Immunology
 
Description Substitution of the -OH group at C-9 of sialic acid by an O-acetyl ester has been suggested to modify various biological phenomena that are regulated by sialic acids. Amongst them, enhancement of erythrocyte lysis by 9-O-acetylated sialic acid determinants through modulation of the alternate pathway of complement has been extensively studied on murine erythrocytes [1]. A variable expression of linkage specific 9-O-acetylated sialoglycoconjugates as defined by the lectinogenic epitope of Achatinin-H namely 9-O-acetylated sialic acid a2!6Gal NAc was identified on rabbit, guinea pig, hamster, rat, mouse and human erythrocytes. This differential expression of linkage specific 9-O-acetylated sialoglycoconjugates strongly correlated with the susceptibility of mammalian erythrocytes to lysis by the alternate pathway of complement. Additionally, low levels of antibodies directed against O-acetylated sialic acids in these mammalian species suggested that these constitutively present determinants have low immunogenicity. Taken together, our results indicate that complement mediated hemolysis depends not simply upon the extent of surface 9-O-acetylated sialic acids present but more importantly upon the specific linkage.
 
Publisher Kluwer
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/828/1/GLYCOCONJUGATE_JOURNAl_17(12)_887%2D893;2000[4].pdf
Sharma, Vineeta and Chatterjee, Mitali and Sen, Goutam and Chava, Anil Kumar and Mandal, Chitra (2000) Role of Linkage Specific 9-O-Acetylated Sialoglycoconjugates in Activation of the Alternate Complement Pathway in Mammalian Erythrocytes. Glycoconjugate Journal, 17 (12). pp. 887-893.
 
Relation http://dx.doi.org/10.1023/A:1010925414222
http://www.eprints.iicb.res.in/828/