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Chromosome profile of Leishmania donovani: Interstrain and interspecific variations

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Chromosome profile of Leishmania donovani: Interstrain and interspecific variations
 
Creator Ghosh, Siddhartha Sankar
Mukherjee, Sandip
Adhya, Samit
 
Subject Molecular & Human Genetics
 
Description The genome of Leishmania donovani AG83, a virulent strain causing kala-azar, was resolved into 29 chromosomal bands by pulsed field gel electrophoresis (pFGE) under standardized conditions. Comparison of the karyotype with those of other strains and species revealed variations. By Southern hybridization, specific genes were localized to individual chromosomes. Twenty-two copies of P-tubulin genes are located on band 27 (1.63 Mb); minor copies are present in band 16 (850 kb) and band 9 (650 kb). A P-tubulin related nontranscribed locus was isolated from a genomic library and shown to contain repetitive sequences hybridizing throughout the genome. Single chromosomes contain multicopy clusters of gp63 and rniniexon- derived RNA genes, but interspecific variations were observed in each case. The results emphasize the importance of using a standard reference strain of Leishmania donovani for coordinated genome mapping of this clinically important organism.
 
Date 1998
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/838/1/JOURNAL_OF_BIOSCIENCES%2C_23(_3)%2C_247%2D254[20].pdf
Ghosh, Siddhartha Sankar and Mukherjee, Sandip and Adhya, Samit (1998) Chromosome profile of Leishmania donovani: Interstrain and interspecific variations. Journal of Bioscience, 23 (3). pp. 247-254.
 
Relation http://dx.doi.org/10.1007/BF02720026
http://www.eprints.iicb.res.in/838/