Ordered Cloned DNA Map of the Genome of Vibrio cholerae 569B and Localization of Genetic Markers
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Ordered Cloned DNA Map of the Genome of Vibrio cholerae
569B and Localization of Genetic Markers
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| Creator |
Chatterjee, Soma
Mondal, Asim K Begum, Nasim A Roychoudhury, Susanta Das, Jyotirmoy |
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| Subject |
Infectious Diseases and Immunology
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| Description |
By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol.
176:1105–1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic strain
569B of Vibrio cholerae has been constructed. A cosmid library the size of about 4,000 clones containing more
than 120 Mb of V. cholerae genomic DNA (40-genome equivalent) was generated. By combining landmark
analysis and chromosome walking, the cosmid clones were assembled into 13 contigs covering about 90% of the
V. cholerae genome. A total of 92 cosmid clones were assigned to the genome and to regions defined by NotI, SfiI, and CeuI macrorestriction maps. Twenty-seven cloned genes, 9 rrn operons, and 10 copies of a repetitive DNA
sequence (IS1004) have been positioned on the ordered cloned DNA map.
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| Date |
1998
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/842/1/JOURNAL_OF_BACTERIOLOGY%2C_180(_4)%2C_901%2D908[57].pdf
Chatterjee, Soma and Mondal, Asim K and Begum, Nasim A and Roychoudhury, Susanta and Das, Jyotirmoy (1998) Ordered Cloned DNA Map of the Genome of Vibrio cholerae 569B and Localization of Genetic Markers. Journal of Bacteriology, 180 (4). pp. 90-908. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/842/ |
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