Vibrio cholerae O139 Bengal: Combined Physical and Genetic Map and Comparative Analysis with the Genome of V. cholerae O1
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Vibrio cholerae O139 Bengal: Combined Physical and Genetic Map and Comparative Analysis with the Genome of V. cholerae O1
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| Creator |
Khetawat, Gopal
Bhadra, Rupak K Kar, Sujata Das, Jyotirmoy |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A combined physical and genetic map of the genome of strain SG24 of Vibrio cholerae O139 Bengal, a novel
non-O1 strain having epidemic potential, has been constructed by using the enzymes NotI, SfiI, and CeuI. The
genome of SG24 is circular, and the genome size is about 3.57 Mb. The linkages between 47 NotI and 32 SfiI
fragments of V. cholerae SG24 genomic DNA were determined by combining two approaches: (i) identification
of fragments produced by enzyme I in fragments produced by enzyme II by the method of fragment excision,
redigestion, and end labeling and (ii) use of the linking clone libraries generated from the genome of classical
O1 strain 569B. The linkages between nine CeuI fragments were determined primarily by analyses of partial
fragments of the CeuI-digested genome. More than 80 cloned homologous and heterologous genes, including
several operons, have been positioned on the physical map. The map of the SG24 genome represents the second
map of a V. cholerae genome, and a comparison of this map with that of classical O1 strain 569B revealed considerable
diversity in DNA restriction sites and allowed identification of hypervariable regions. Several genetic
markers, including virulence determinant genes, are in different positions in the SG24 and 569B genomes.
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| Date |
1998
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/844/1/JOURNAL_OF_BACTERIOLOGY%2C180(_17)%2C_4516%2D4522[22].pdf
Khetawat, Gopal and Bhadra, Rupak K and Kar, Sujata and Das, Jyotirmoy (1998) Vibrio cholerae O139 Bengal: Combined Physical and Genetic Map and Comparative Analysis with the Genome of V. cholerae O1. Journal of Bacteriology, 180 (17). pp. 4516-4522. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/844/ |
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