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Termitomyces Clypeatus Controls Secretion of Extracellular Amyloglucosidase by Regulating Exocytosis of Vacuolar Enzyme

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Termitomyces Clypeatus Controls Secretion of Extracellular Amyloglucosidase by Regulating Exocytosis of Vacuolar Enzyme
 
Creator Sengupta, Trishna
Hazra, P P
Mukhopadhyay, A
Sengupta, S
 
Subject Chemistry
 
Description An extracellular amyloglucosidase (56 kDa) of Termitomyces clypeatus which was accumulated intracellularly in absence of Krebs cycle acids in dextrin medium, remained mostly inside the plasma membrane. The enzyme was localised in mycelial vacuoles as a sucrase-amyloglucosidase aggregate. The intracellular pool contained both the free sucrase and the aggregate, while vacuoles contained only aggregate without any free enzyme. The aggregate, partially purified to PAGE homogeneity, contained amyloglucosidase and sucrase in the ratio of 1:1. A cellular regulation, influenced by the presence of Krebs cycle acids, was indicated to be present at the level of exocytosis of vacuolar enzyme.
 
Date 1998
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/852/1/FEMS_MICROBIOLOGY_LETTERS%2C_158(_1)%2C_101%2D105[68].pdf
Sengupta, Trishna and Hazra, P P and Mukhopadhyay, A and Sengupta, S (1998) Termitomyces Clypeatus Controls Secretion of Extracellular Amyloglucosidase by Regulating Exocytosis of Vacuolar Enzyme. FEMS Microbiology Letters, 158 (1). pp. 101-105.
 
Relation http://dx.doi.org/10.1016/S0378-1097(97)00507-7
http://www.eprints.iicb.res.in/852/